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http://purl.uniprot.org/citations/19368703http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/19368703http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/19368703http://www.w3.org/2000/01/rdf-schema#comment"

Background

Multiple sclerosis-associated retrovirus (MSRV) RNA sequences have been detected in patients with multiple sclerosis (MS) and are related to the multi-copy human endogenous retrovirus family type W (HERV-W). Only one HERV-W locus (ERVWE1) codes for a complete HERV-W Env protein (Syncytin-1). Syncytin-1 and the putative MSRV Env protein have been involved in the pathogenesis of MS. The origin of MSRV and its precise relation to HERV-W were hitherto unknown.

Results

By mapping HERV-W env cDNA sequences (n = 332) from peripheral blood mononuclear cells of patients with MS and healthy controls onto individual genomic HERV-W env elements, we identified seven transcribed HERV-W env loci in these cells, including ERVWE1. Transcriptional activity of individual HERV-W env elements did not significantly differ between patients with MS and controls. Remarkably, almost 30% of HERV-W env cDNAs were recombined sequences that most likely arose in vitro between transcripts from different HERV-W env elements. Re-analysis of published MSRV env sequences revealed that all of them can be explained as originating from genomic HERV-W env loci or recombinations among them. In particular, a MSRV env clone previously used for the generation of monoclonal antibody 6A2B2, detecting an antigen in MS brain lesions, appears to be derived from a HERV-W env locus on chromosome Xq22.3. This locus harbors a long open reading frame for an N-terminally truncated HERV-W Env protein.

Conclusion

Our data clarify the origin of MSRV env sequences, have important implications for the status of MSRV, and open the possibility that a protein encoded by a HERV-W env element on chromosome Xq22.3 may be expressed in MS brain lesions."xsd:string
http://purl.uniprot.org/citations/19368703http://purl.org/dc/terms/identifier"doi:10.1186/1742-4690-6-37"xsd:string
http://purl.uniprot.org/citations/19368703http://purl.uniprot.org/core/author"Mueller-Lantzsch N."xsd:string
http://purl.uniprot.org/citations/19368703http://purl.uniprot.org/core/author"Mueller-Lantzsch N."xsd:string
http://purl.uniprot.org/citations/19368703http://purl.uniprot.org/core/author"Mayer J."xsd:string
http://purl.uniprot.org/citations/19368703http://purl.uniprot.org/core/author"Mayer J."xsd:string
http://purl.uniprot.org/citations/19368703http://purl.uniprot.org/core/author"Laufer G."xsd:string
http://purl.uniprot.org/citations/19368703http://purl.uniprot.org/core/author"Laufer G."xsd:string
http://purl.uniprot.org/citations/19368703http://purl.uniprot.org/core/author"Mueller B.F."xsd:string
http://purl.uniprot.org/citations/19368703http://purl.uniprot.org/core/author"Mueller B.F."xsd:string
http://purl.uniprot.org/citations/19368703http://purl.uniprot.org/core/author"Ruprecht K."xsd:string
http://purl.uniprot.org/citations/19368703http://purl.uniprot.org/core/author"Ruprecht K."xsd:string
http://purl.uniprot.org/citations/19368703http://purl.uniprot.org/core/date"2009"xsd:gYear
http://purl.uniprot.org/citations/19368703http://purl.uniprot.org/core/date"2009"xsd:gYear
http://purl.uniprot.org/citations/19368703http://purl.uniprot.org/core/name"Retrovirology"xsd:string
http://purl.uniprot.org/citations/19368703http://purl.uniprot.org/core/name"Retrovirology"xsd:string
http://purl.uniprot.org/citations/19368703http://purl.uniprot.org/core/pages"37"xsd:string
http://purl.uniprot.org/citations/19368703http://purl.uniprot.org/core/pages"37"xsd:string
http://purl.uniprot.org/citations/19368703http://purl.uniprot.org/core/title"Analysis of transcribed human endogenous retrovirus W env loci clarifies the origin of multiple sclerosis-associated retrovirus env sequences."xsd:string
http://purl.uniprot.org/citations/19368703http://purl.uniprot.org/core/title"Analysis of transcribed human endogenous retrovirus W env loci clarifies the origin of multiple sclerosis-associated retrovirus env sequences."xsd:string
http://purl.uniprot.org/citations/19368703http://purl.uniprot.org/core/volume"6"xsd:string
http://purl.uniprot.org/citations/19368703http://purl.uniprot.org/core/volume"6"xsd:string
http://purl.uniprot.org/citations/19368703http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/19368703
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