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| Subject | Predicate | Object |
|---|---|---|
| http://purl.uniprot.org/citations/19376894 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19376894 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19376894 | http://www.w3.org/2000/01/rdf-schema#comment | "We determined the effect of the host on the function and structure of the nearly identical IncP-7 carbazole-degradative plasmids pCAR1.1 and pCAR1.2. We constructed Pseudomonas aeruginosa PAO1(pCAR1.2) and P. fluorescens Pf0-1Km(pCAR1.2) and compared their growth on carbazole- and succinate-containing media with that of P. putida KT2440(pCAR1.1). We also assessed the stability of the genetic structures of the plasmids in each of the three hosts. Pf0-1Km(pCAR1.2) showed dramatically delayed growth when carbazole was supplied as the sole carbon source, while the three strains grew at nearly the same rate on succinate. Among the carbazole-grown Pf0-1Km(pCAR1.2) cells, two types of deficient strains appeared and dominated the population; such dominance was not observed in the other two strains or for succinate-grown Pf0-1Km(pCAR1.2). Genetic analysis showed that the two deficient strains possessed pCAR1.2 derivatives in which the carbazole-degradative car operon was deleted or its regulatory gene, antR, was deleted by homologous recombination between insertion sequences. From genomic information and quantitative reverse transcription-PCR analyses of the genes involved in carbazole mineralization by Pf0-1Km(pCAR1.2), we found that the cat genes on the chromosome of Pf0-1Km, which are necessary for the degradation of catechol (a toxic intermediate in the carbazole catabolic pathway), were not induced in the presence of carbazole. The resulting accumulation of catechol may have enabled the strain that lost its carbazole-degrading ability to have overall higher fitness than the wild-type strain. These results suggest that the functions of the chromosomal genes contributed to the selection of plasmid derivatives with altered structures."xsd:string |
| http://purl.uniprot.org/citations/19376894 | http://purl.org/dc/terms/identifier | "doi:10.1128/aem.02373-08"xsd:string |
| http://purl.uniprot.org/citations/19376894 | http://purl.org/dc/terms/identifier | "doi:10.1128/aem.02373-08"xsd:string |
| http://purl.uniprot.org/citations/19376894 | http://purl.org/dc/terms/identifier | "doi:10.1128/AEM.02373-08"xsd:string |
| http://purl.uniprot.org/citations/19376894 | http://purl.uniprot.org/core/author | "Li L."xsd:string |
| http://purl.uniprot.org/citations/19376894 | http://purl.uniprot.org/core/author | "Li L."xsd:string |
| http://purl.uniprot.org/citations/19376894 | http://purl.uniprot.org/core/author | "Takahashi Y."xsd:string |
| http://purl.uniprot.org/citations/19376894 | http://purl.uniprot.org/core/author | "Takahashi Y."xsd:string |
| http://purl.uniprot.org/citations/19376894 | http://purl.uniprot.org/core/author | "Yamane H."xsd:string |
| http://purl.uniprot.org/citations/19376894 | http://purl.uniprot.org/core/author | "Yamane H."xsd:string |
| http://purl.uniprot.org/citations/19376894 | http://purl.uniprot.org/core/author | "Nojiri H."xsd:string |
| http://purl.uniprot.org/citations/19376894 | http://purl.uniprot.org/core/author | "Nojiri H."xsd:string |
| http://purl.uniprot.org/citations/19376894 | http://purl.uniprot.org/core/author | "Shintani M."xsd:string |
| http://purl.uniprot.org/citations/19376894 | http://purl.uniprot.org/core/author | "Shintani M."xsd:string |
| http://purl.uniprot.org/citations/19376894 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19376894 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19376894 | http://purl.uniprot.org/core/name | "Appl. Environ. Microbiol."xsd:string |
| http://purl.uniprot.org/citations/19376894 | http://purl.uniprot.org/core/name | "Appl. Environ. Microbiol."xsd:string |
| http://purl.uniprot.org/citations/19376894 | http://purl.uniprot.org/core/pages | "3920-3929"xsd:string |
| http://purl.uniprot.org/citations/19376894 | http://purl.uniprot.org/core/pages | "3920-3929"xsd:string |
| http://purl.uniprot.org/citations/19376894 | http://purl.uniprot.org/core/title | "Carbazole-degradative IncP-7 plasmid pCAR1.2 is structurally unstable in Pseudomonas fluorescens Pf0-1, which accumulates catechol, the intermediate of the carbazole degradation pathway."xsd:string |
| http://purl.uniprot.org/citations/19376894 | http://purl.uniprot.org/core/title | "Carbazole-degradative IncP-7 plasmid pCAR1.2 is structurally unstable in Pseudomonas fluorescens Pf0-1, which accumulates catechol, the intermediate of the carbazole degradation pathway."xsd:string |
| http://purl.uniprot.org/citations/19376894 | http://purl.uniprot.org/core/volume | "75"xsd:string |