http://purl.uniprot.org/citations/19392987 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/19392987 | http://www.w3.org/2000/01/rdf-schema#comment | "BackgroundIn many patients with atopic dermatitis (AD), the disease is complicated by their enhanced susceptibility to bacterial skin infections, especially with Staphylococcus aureus. The pattern recognition receptor toll-like receptor (TLR)-2 recognizes components of S. aureus, for example, lipoteichoic acid (LTA) and peptidoglycan (PGN) and, therefore, might be crucial in the pathogenesis and flare-ups of AD.ObjectiveTo investigate TLR-2 expression and cytokine secretion in macrophages from patients with AD compared to healthy controls upon TLR-2 stimulation with PGN, LTA and Pam3Cys.MethodsMacrophages were cultivated from highly purified peripheral blood monocytes of AD patients and nonatopic healthy controls and stimulated with PGN, LTA and Pam3Cys in a time and dose-dependent manner. Afterwards, TLR-2 expression and cytokine secretion were measured on protein and mRNA level. TLR-1 and TLR-6 expression were investigated on the mRNA level. Immunohistochemical stainings from punch biopsies were performed to investigate TLR-2 expression in skin macrophages.ResultsWe could clearly show that macrophages from patients with AD expressed significantly less TLR-2, whereas the expression pattern of TLR-1 and TLR-6 were not altered. Macrophages had a reduced capacity to produce pro-inflammatory cytokines such as IL-6, IL-8 and IL-1beta after stimulation with TLR-2 ligands.ConclusionOur findings clearly show an impaired TLR-2 expression and functional differences of TLR-2-mediated effects on macrophages of AD patients compared to healthy controls which might contribute to the enhanced susceptibility to skin infections with S. aureus in AD."xsd:string |
http://purl.uniprot.org/citations/19392987 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1398-9995.2009.02050.x"xsd:string |
http://purl.uniprot.org/citations/19392987 | http://purl.uniprot.org/core/author | "Niebuhr M."xsd:string |
http://purl.uniprot.org/citations/19392987 | http://purl.uniprot.org/core/author | "Werfel T."xsd:string |
http://purl.uniprot.org/citations/19392987 | http://purl.uniprot.org/core/author | "Lutat C."xsd:string |
http://purl.uniprot.org/citations/19392987 | http://purl.uniprot.org/core/author | "Sigel S."xsd:string |
http://purl.uniprot.org/citations/19392987 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
http://purl.uniprot.org/citations/19392987 | http://purl.uniprot.org/core/name | "Allergy"xsd:string |
http://purl.uniprot.org/citations/19392987 | http://purl.uniprot.org/core/pages | "1580-1587"xsd:string |
http://purl.uniprot.org/citations/19392987 | http://purl.uniprot.org/core/title | "Impaired TLR-2 expression and TLR-2-mediated cytokine secretion in macrophages from patients with atopic dermatitis."xsd:string |
http://purl.uniprot.org/citations/19392987 | http://purl.uniprot.org/core/volume | "64"xsd:string |
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