| http://purl.uniprot.org/citations/19401205 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19401205 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19401205 | http://www.w3.org/2000/01/rdf-schema#comment | "Heat shock protein 90 (Hsp-90) is a highly conserved essential protein in eukaryotes. Here we describe the molecular characterisation of hsp-90 from three nematodes, the free-living Caenorhabditis elegans (Ce) and the parasitic worms Brugia pahangi (Bp) and Haemonchus contortus (Hc). These molecules were functionally characterised by rescue of a Ce-daf-21 (hsp-90) null mutant. Our results show a gradient of rescue: the C. elegans endogenous gene provided full rescue of the daf-21 mutant, while Hc-hsp-90 provided partial rescue. In contrast, no rescue could be obtained using a variety of Bp-hsp-90 constructs, despite the fact that Bp-hsp-90 was transcribed and translated in the mutant worms. daf-21 RNA interference (RNAi) experiments were carried out to determine whether knock-down of the endogenous daf-21 mRNA in N2 worms could be complemented by expression of either parasite gene. However neither parasite gene could rescue the daf-21 (RNAi) phenotypes. These results indicate that factors other than the level of sequence identity are important for determining whether parasite genes can functionally complement in C. elegans."xsd:string |
| http://purl.uniprot.org/citations/19401205 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.ijpara.2009.02.024"xsd:string |
| http://purl.uniprot.org/citations/19401205 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.ijpara.2009.02.024"xsd:string |
| http://purl.uniprot.org/citations/19401205 | http://purl.uniprot.org/core/author | "Devaney E."xsd:string |
| http://purl.uniprot.org/citations/19401205 | http://purl.uniprot.org/core/author | "Devaney E."xsd:string |
| http://purl.uniprot.org/citations/19401205 | http://purl.uniprot.org/core/author | "McCormack G."xsd:string |
| http://purl.uniprot.org/citations/19401205 | http://purl.uniprot.org/core/author | "McCormack G."xsd:string |
| http://purl.uniprot.org/citations/19401205 | http://purl.uniprot.org/core/author | "Maitland K."xsd:string |
| http://purl.uniprot.org/citations/19401205 | http://purl.uniprot.org/core/author | "Maitland K."xsd:string |
| http://purl.uniprot.org/citations/19401205 | http://purl.uniprot.org/core/author | "Gillan V."xsd:string |
| http://purl.uniprot.org/citations/19401205 | http://purl.uniprot.org/core/author | "Gillan V."xsd:string |
| http://purl.uniprot.org/citations/19401205 | http://purl.uniprot.org/core/author | "Him N.A."xsd:string |
| http://purl.uniprot.org/citations/19401205 | http://purl.uniprot.org/core/author | "Him N.A."xsd:string |
| http://purl.uniprot.org/citations/19401205 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19401205 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19401205 | http://purl.uniprot.org/core/name | "Int. J. Parasitol."xsd:string |
| http://purl.uniprot.org/citations/19401205 | http://purl.uniprot.org/core/name | "Int J Parasitol"xsd:string |
| http://purl.uniprot.org/citations/19401205 | http://purl.uniprot.org/core/pages | "1071-1081"xsd:string |
| http://purl.uniprot.org/citations/19401205 | http://purl.uniprot.org/core/pages | "1071-1081"xsd:string |
| http://purl.uniprot.org/citations/19401205 | http://purl.uniprot.org/core/title | "Functional genomics of hsp-90 in parasitic and free-living nematodes."xsd:string |
| http://purl.uniprot.org/citations/19401205 | http://purl.uniprot.org/core/title | "Functional genomics of hsp-90 in parasitic and free-living nematodes."xsd:string |
| http://purl.uniprot.org/citations/19401205 | http://purl.uniprot.org/core/volume | "39"xsd:string |
| http://purl.uniprot.org/citations/19401205 | http://purl.uniprot.org/core/volume | "39"xsd:string |