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http://purl.uniprot.org/citations/19403154http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/19403154http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/19403154http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/19403154http://www.w3.org/2000/01/rdf-schema#comment"We report the discovery of a new virus from the red imported fire ant, Solenopsis invicta. Solenopsis invicta virus 3 (SINV-3) represents the third virus discovered from this ant species using the metagenomics approach. The single (positive)-strand RNA, monopartite, bicistronic genome of SINV-3 was sequenced in entirety (GenBank accession number FJ528584), comprised of 10,386 nucleotides, and polyadenylated at the 3' terminus. This genome size was confirmed by Northern analysis. The genome revealed 2 large open reading frames (ORFs) in the sense orientation with an untranslated region (UTR) at each end and between the two ORFs. The 5' proximal ORF (ORF 1) encoded a predicted protein of 299.1 kDa (2580 amino acids). The 3' proximal ORF (ORF 2) encoded a predicted protein of 73.2 kDa (651 amino acids). RNA-dependent RNA polymerase (RdRp), helicase, and protease domains were recognized in ORF 1. SDS-PAGE separation of purified SINV-3 particles yielded 2 bands (ostensibly capsid proteins) with a combined molecular mass of 77.3 kDa which was similar to the mass predicted by ORF 2 (73.2 kDa). Phylogenetic analysis of the conserved amino acid sequences containing domains I to VIII of the RdRp from dicistroviruses, iflaviruses, plant small RNA viruses, picornaviruses, and 4 unassigned positive-strand RNA viruses revealed a trichotomous phenogram with SINV-3 and Kelp fly virus comprising a unique cluster. Electron microscopic examination of negatively stained samples of SINV-3 revealed isometric particles with apparent projections and a diameter of 27.3+/-1.3 nm. SINV-3 was successfully transmitted to uninfected workers by feeding. The minus (replicative) strand of SINV-3 was detected in worker ants indicating replication of the virus. The possibility of using SINV-3 as a microbial control agent for fire ants is discussed."xsd:string
http://purl.uniprot.org/citations/19403154http://purl.org/dc/terms/identifier"doi:10.1016/j.virol.2009.03.028"xsd:string
http://purl.uniprot.org/citations/19403154http://purl.org/dc/terms/identifier"doi:10.1016/j.virol.2009.03.028"xsd:string
http://purl.uniprot.org/citations/19403154http://purl.org/dc/terms/identifier"doi:10.1016/j.virol.2009.03.028"xsd:string
http://purl.uniprot.org/citations/19403154http://purl.uniprot.org/core/author"Hashimoto Y."xsd:string
http://purl.uniprot.org/citations/19403154http://purl.uniprot.org/core/author"Hashimoto Y."xsd:string
http://purl.uniprot.org/citations/19403154http://purl.uniprot.org/core/author"Hashimoto Y."xsd:string
http://purl.uniprot.org/citations/19403154http://purl.uniprot.org/core/author"Valles S.M."xsd:string
http://purl.uniprot.org/citations/19403154http://purl.uniprot.org/core/author"Valles S.M."xsd:string
http://purl.uniprot.org/citations/19403154http://purl.uniprot.org/core/author"Valles S.M."xsd:string
http://purl.uniprot.org/citations/19403154http://purl.uniprot.org/core/date"2009"xsd:gYear
http://purl.uniprot.org/citations/19403154http://purl.uniprot.org/core/date"2009"xsd:gYear
http://purl.uniprot.org/citations/19403154http://purl.uniprot.org/core/date"2009"xsd:gYear
http://purl.uniprot.org/citations/19403154http://purl.uniprot.org/core/name"Virology"xsd:string
http://purl.uniprot.org/citations/19403154http://purl.uniprot.org/core/name"Virology"xsd:string
http://purl.uniprot.org/citations/19403154http://purl.uniprot.org/core/name"Virology"xsd:string
http://purl.uniprot.org/citations/19403154http://purl.uniprot.org/core/pages"354-361"xsd:string
http://purl.uniprot.org/citations/19403154http://purl.uniprot.org/core/pages"354-361"xsd:string
http://purl.uniprot.org/citations/19403154http://purl.uniprot.org/core/pages"354-361"xsd:string
http://purl.uniprot.org/citations/19403154http://purl.uniprot.org/core/title"Isolation and characterization of Solenopsis invicta virus 3, a new positive-strand RNA virus infecting the red imported fire ant, Solenopsis invicta."xsd:string
http://purl.uniprot.org/citations/19403154http://purl.uniprot.org/core/title"Isolation and characterization of Solenopsis invicta virus 3, a new positive-strand RNA virus infecting the red imported fire ant, Solenopsis invicta."xsd:string
http://purl.uniprot.org/citations/19403154http://purl.uniprot.org/core/title"Isolation and characterization of Solenopsis invicta virus 3, a new positive-strand RNA virus infecting the red imported fire ant, Solenopsis invicta."xsd:string
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