| http://purl.uniprot.org/citations/19429681 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19429681 | http://www.w3.org/2000/01/rdf-schema#comment | "Clostridium perfringens enterotoxin (CPE) binds to the extracellular loop 2 of a subset of claudins, e.g. claudin-3. Here, the molecular mechanism of the CPE-claudin interaction was analyzed. Using peptide arrays, recombinant CPE-(116-319) bound to loop 2 peptides of mouse claudin-3, -6, -7, -9, and -14 but not of 1, 2, 4, 5, 8, 10-13, 15, 16, 18-20, and 22. Substitution peptide mapping identified the central motif (148)NPL(150)VP, supposed to represent a turn region in the loop 2, as essential for the interaction between CPE and murine claudin-3 peptides. CPE-binding assays with claudin-3 mutant-transfected HEK293 cells or lysates thereof demonstrated the involvement of Asn(148) and Leu(150) of full-length claudin-3 in the binding. CPE-(116-319) and CPE-(194-319) bound to HEK293 cells expressing claudin-3, whereas CPE-(116-319) bound to claudin-5-expressing HEK293 cells, also. This binding was inhibited by substitutions T151A and Q156E in claudin-5. In contrast, removal of the aromatic side chains in the loop 2 of claudin-3 and -5, involved in trans-interaction between claudins, increased the amount of CPE-(116-319) bound. These findings and molecular modeling indicate different molecular mechanisms of claudin-claudin trans-interaction and claudin-CPE interaction. Confocal microscopy showed that CPE-(116-319) and CPE-(194-319) bind to claudin-3 at the plasma membrane, outside cell-cell contacts. Together, these findings demonstrate that CPE binds to the hydrophobic turn and flanking polar residues in the loop 2 of claudin-3 outside tight junctions. The data can be used for the specific design of CPE-based modulators of tight junctions, to improve drug delivery, and as chemotherapeutics for tumors overexpressing claudins."xsd:string |
| http://purl.uniprot.org/citations/19429681 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m109.008623"xsd:string |
| http://purl.uniprot.org/citations/19429681 | http://purl.uniprot.org/core/author | "Krause G."xsd:string |
| http://purl.uniprot.org/citations/19429681 | http://purl.uniprot.org/core/author | "Gehring C."xsd:string |
| http://purl.uniprot.org/citations/19429681 | http://purl.uniprot.org/core/author | "Wenzel A."xsd:string |
| http://purl.uniprot.org/citations/19429681 | http://purl.uniprot.org/core/author | "Piontek J."xsd:string |
| http://purl.uniprot.org/citations/19429681 | http://purl.uniprot.org/core/author | "Winkler L."xsd:string |
| http://purl.uniprot.org/citations/19429681 | http://purl.uniprot.org/core/author | "Blasig I.E."xsd:string |
| http://purl.uniprot.org/citations/19429681 | http://purl.uniprot.org/core/author | "Piehl C."xsd:string |
| http://purl.uniprot.org/citations/19429681 | http://purl.uniprot.org/core/author | "Muller S.L."xsd:string |
| http://purl.uniprot.org/citations/19429681 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19429681 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/19429681 | http://purl.uniprot.org/core/pages | "18863-18872"xsd:string |
| http://purl.uniprot.org/citations/19429681 | http://purl.uniprot.org/core/title | "Molecular determinants of the interaction between Clostridium perfringens enterotoxin fragments and claudin-3."xsd:string |
| http://purl.uniprot.org/citations/19429681 | http://purl.uniprot.org/core/volume | "284"xsd:string |
| http://purl.uniprot.org/citations/19429681 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19429681 |
| http://purl.uniprot.org/citations/19429681 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19429681 |
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