| http://purl.uniprot.org/citations/19433064 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19433064 | http://www.w3.org/2000/01/rdf-schema#comment | "Extracellular Ca2+ entry, primarily mediated through store-operated Ca2+ entry (SOCE), is known to be a critical event for NADPH oxidase (NOX2) regulation in neutrophils. While defective NOX2 activity has been linked to various inflammatory diseases, regulatory mechanisms that control Ca2+ influx-induced NOX2 activation are poorly understood in SOCE. The role of STIM1, a Ca2+ sensor that transduces the store depletion signal to the plasma membrane, seems well established and supported by numerous studies in non-phagocytic cells. Here, in neutrophil-like HL-60 cells we used a siRNA approach to delineate the effect of STIM1 knock-down on NOX2 activity regulated by Ca2+ influx. Because the function of the STIM1 homolog, STIM2, is still unclear, we determined the consequence of STIM2 knock-down on Ca2+ and NOX2. STIM1 and STIM2 knock-down was effective and isoform specific when assayed by real-time PCR and Western blotting. Consistent with a unique role of STIM1 in the regulation of SOCE, STIM1, but not STIM2, siRNA significantly decreased Ca2+ influx induced by fMLF or the SERCA pump inhibitor thapsigargin. A redistribution of STIM1, originally localized intracellularly, near the plasma membrane was observed by confocal microscopy upon stimulation by fMLF. Inhibition of STIM1-induced SOCE led to a marked decrease in NOX2 activity while STIM2 siRNA had no effect. Thus, our results provide evidence for a role of STIM1 protein in the control of Ca2+ influx in neutrophils excluding a STIM2 involvement in this process. It also places STIM1 as a key modulator of NOX2 activity with a potential interest for anti-inflammatory pharmacological development."xsd:string |
| http://purl.uniprot.org/citations/19433064 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bcp.2009.05.006"xsd:string |
| http://purl.uniprot.org/citations/19433064 | http://purl.uniprot.org/core/author | "Melchior C."xsd:string |
| http://purl.uniprot.org/citations/19433064 | http://purl.uniprot.org/core/author | "Plancon S."xsd:string |
| http://purl.uniprot.org/citations/19433064 | http://purl.uniprot.org/core/author | "Brechard S."xsd:string |
| http://purl.uniprot.org/citations/19433064 | http://purl.uniprot.org/core/author | "Tschirhart E.J."xsd:string |
| http://purl.uniprot.org/citations/19433064 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19433064 | http://purl.uniprot.org/core/name | "Biochem Pharmacol"xsd:string |
| http://purl.uniprot.org/citations/19433064 | http://purl.uniprot.org/core/pages | "504-513"xsd:string |
| http://purl.uniprot.org/citations/19433064 | http://purl.uniprot.org/core/title | "STIM1 but not STIM2 is an essential regulator of Ca2+ influx-mediated NADPH oxidase activity in neutrophil-like HL-60 cells."xsd:string |
| http://purl.uniprot.org/citations/19433064 | http://purl.uniprot.org/core/volume | "78"xsd:string |
| http://purl.uniprot.org/citations/19433064 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19433064 |
| http://purl.uniprot.org/citations/19433064 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19433064 |
| http://purl.uniprot.org/uniprot/#_G0XQ39-mappedCitation-19433064 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19433064 |
| http://purl.uniprot.org/uniprot/#_B3KUB5-mappedCitation-19433064 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19433064 |
| http://purl.uniprot.org/uniprot/#_H0Y860-mappedCitation-19433064 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19433064 |
| http://purl.uniprot.org/uniprot/#_Q13586-mappedCitation-19433064 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19433064 |
| http://purl.uniprot.org/uniprot/#_Q05BJ5-mappedCitation-19433064 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19433064 |
| http://purl.uniprot.org/uniprot/#_Q9P246-mappedCitation-19433064 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19433064 |
| http://purl.uniprot.org/uniprot/#_V5J3L2-mappedCitation-19433064 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19433064 |
| http://purl.uniprot.org/uniprot/H0Y860 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19433064 |
| http://purl.uniprot.org/uniprot/Q13586 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19433064 |
| http://purl.uniprot.org/uniprot/G0XQ39 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19433064 |
| http://purl.uniprot.org/uniprot/Q9P246 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19433064 |