| http://purl.uniprot.org/citations/19445977 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19445977 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19445977 | http://www.w3.org/2000/01/rdf-schema#comment | "A gene library is a useful tool for molecular biology studies, but the classical screening of multiple plates is laborious and time-consuming. Cosmid gene libraries are particularly well suited for isolation of large gene clusters encoding the biosynthetic pathways of secondary metabolites in Streptomyces. A gene library of the immunosuppressant tacrolimus-producer strain Streptomyces sp. ATCC 55098 was constructed in the SuperCos1 vector and 1656 clones were organized in an easy pyramidal arrangement system. This clustering method allows a dual efficient screening (PCR and in situ colony hybridization) of the gene library in a two-step method by using only one 96-well plate. The dual screening combines the advantages of both techniques, the swiftness of PCR and the robustness of colony hybridization."xsd:string |
| http://purl.uniprot.org/citations/19445977 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.mimet.2009.05.005"xsd:string |
| http://purl.uniprot.org/citations/19445977 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.mimet.2009.05.005"xsd:string |
| http://purl.uniprot.org/citations/19445977 | http://purl.uniprot.org/core/author | "Martin J.F."xsd:string |
| http://purl.uniprot.org/citations/19445977 | http://purl.uniprot.org/core/author | "Martin J.F."xsd:string |
| http://purl.uniprot.org/citations/19445977 | http://purl.uniprot.org/core/author | "Barreiro C."xsd:string |
| http://purl.uniprot.org/citations/19445977 | http://purl.uniprot.org/core/author | "Barreiro C."xsd:string |
| http://purl.uniprot.org/citations/19445977 | http://purl.uniprot.org/core/author | "Martinez-Castro M."xsd:string |
| http://purl.uniprot.org/citations/19445977 | http://purl.uniprot.org/core/author | "Martinez-Castro M."xsd:string |
| http://purl.uniprot.org/citations/19445977 | http://purl.uniprot.org/core/author | "Solera E."xsd:string |
| http://purl.uniprot.org/citations/19445977 | http://purl.uniprot.org/core/author | "Solera E."xsd:string |
| http://purl.uniprot.org/citations/19445977 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19445977 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19445977 | http://purl.uniprot.org/core/name | "J. Microbiol. Methods"xsd:string |
| http://purl.uniprot.org/citations/19445977 | http://purl.uniprot.org/core/name | "J Microbiol Methods"xsd:string |
| http://purl.uniprot.org/citations/19445977 | http://purl.uniprot.org/core/pages | "150-154"xsd:string |
| http://purl.uniprot.org/citations/19445977 | http://purl.uniprot.org/core/pages | "150-154"xsd:string |
| http://purl.uniprot.org/citations/19445977 | http://purl.uniprot.org/core/title | "Efficient pyramidal arrangement of an ordered cosmid library: Rapid screening of genes of the tacrolimus-producer Streptomyces sp. ATCC 55098."xsd:string |
| http://purl.uniprot.org/citations/19445977 | http://purl.uniprot.org/core/title | "Efficient pyramidal arrangement of an ordered cosmid library: Rapid screening of genes of the tacrolimus-producer Streptomyces sp. ATCC 55098."xsd:string |
| http://purl.uniprot.org/citations/19445977 | http://purl.uniprot.org/core/volume | "78"xsd:string |
| http://purl.uniprot.org/citations/19445977 | http://purl.uniprot.org/core/volume | "78"xsd:string |
| http://purl.uniprot.org/citations/19445977 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19445977 |
| http://purl.uniprot.org/citations/19445977 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19445977 |