| http://purl.uniprot.org/citations/19451552 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19451552 | http://www.w3.org/2000/01/rdf-schema#comment | "CD4(+) T cells use the chemokine receptor CCR7 to home to and migrate within lymphoid tissue, where T-cell activation takes place. Using primary T-cell receptor (TCR)-transgenic (tg) CD4(+) T cells, we explored the effect of CCR7 ligands, in particular CCL21, on T-cell activation. We found that the presence of CCL21 during early time points strongly increased in vitro T-cell proliferation after TCR stimulation, correlating with increased expression of early activation markers. CCL21 costimulation resulted in increased Ras- and Rac-GTP formation and enhanced phosphorylation of Akt, MEK, and ERK but not p38 or JNK. Kinase-dead PI3Kdelta(D910A/D910A) or PI3Kgamma-deficient TCR-tg CD4(+) T cells showed similar responsiveness to CCL21 costimulation as control CD4(+) T cells. Conversely, deficiency in the Rac guanine exchange factor DOCK2 significantly impaired CCL21-mediated costimulation in TCR-tg CD4(+) T cells, concomitant with impaired Rac-but not Ras-GTP formation. Using lymph node slices for live monitoring of T-cell behavior and activation, we found that G protein-coupled receptor signaling was required for early CD69 expression but not for Ca(2+) signaling. Our data suggest that the presence of CCL21 during early TCR signaling lowers the activation threshold through Ras- and Rac-dependent pathways leading to increased ERK phosphorylation."xsd:string |
| http://purl.uniprot.org/citations/19451552 | http://purl.org/dc/terms/identifier | "doi:10.1182/blood-2009-01-200923"xsd:string |
| http://purl.uniprot.org/citations/19451552 | http://purl.uniprot.org/core/author | "Fukui Y."xsd:string |
| http://purl.uniprot.org/citations/19451552 | http://purl.uniprot.org/core/author | "Tanaka Y."xsd:string |
| http://purl.uniprot.org/citations/19451552 | http://purl.uniprot.org/core/author | "Peterson J.R."xsd:string |
| http://purl.uniprot.org/citations/19451552 | http://purl.uniprot.org/core/author | "Stein J.V."xsd:string |
| http://purl.uniprot.org/citations/19451552 | http://purl.uniprot.org/core/author | "Donnadieu E."xsd:string |
| http://purl.uniprot.org/citations/19451552 | http://purl.uniprot.org/core/author | "Vanhaesebroeck B."xsd:string |
| http://purl.uniprot.org/citations/19451552 | http://purl.uniprot.org/core/author | "Okkenhaug K."xsd:string |
| http://purl.uniprot.org/citations/19451552 | http://purl.uniprot.org/core/author | "Asperti-Boursin F."xsd:string |
| http://purl.uniprot.org/citations/19451552 | http://purl.uniprot.org/core/author | "Gollmer K."xsd:string |
| http://purl.uniprot.org/citations/19451552 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19451552 | http://purl.uniprot.org/core/name | "Blood"xsd:string |
| http://purl.uniprot.org/citations/19451552 | http://purl.uniprot.org/core/pages | "580-588"xsd:string |
| http://purl.uniprot.org/citations/19451552 | http://purl.uniprot.org/core/title | "CCL21 mediates CD4+ T-cell costimulation via a DOCK2/Rac-dependent pathway."xsd:string |
| http://purl.uniprot.org/citations/19451552 | http://purl.uniprot.org/core/volume | "114"xsd:string |
| http://purl.uniprot.org/citations/19451552 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19451552 |
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| http://purl.uniprot.org/uniprot/#_B1AS25-mappedCitation-19451552 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19451552 |
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| http://purl.uniprot.org/uniprot/#_B1AS27-mappedCitation-19451552 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19451552 |