| http://purl.uniprot.org/citations/19479054 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19479054 | http://www.w3.org/2000/01/rdf-schema#comment | "BackgroundThe molecular pathway that controls cardiogenesis is temporally and spatially regulated by master transcriptional regulators such as NKX2-5, Isl1, MEF2C, GATA4, and beta-catenin. The interplay between these factors and their downstream targets are not completely understood. Here, we studied regulation of beta-catenin and GATA4 by NKX2-5 in human fetal cardiac myocytes.Methodology/principal findingsUsing antisense inhibition we disrupted the expression of NKX2-5 and studied changes in expression of cardiac-associated genes. Down-regulation of NKX2-5 resulted in increased beta-catenin while GATA4 was decreased. We demonstrated that this regulation was conferred by binding of NKX2-5 to specific elements (NKEs) in the promoter region of the beta-catenin and GATA4 genes. Using promoter-luciferase reporter assay combined with mutational analysis of the NKEs we demonstrated that the identified NKX2-5 binding sites were essential for the suppression of beta-catenin, and upregulation of GATA4 by NKX2-5.ConclusionsThis study suggests that NKX2-5 modulates the beta-catenin and GATA4 transcriptional activities in developing human cardiac myocytes."xsd:string |
| http://purl.uniprot.org/citations/19479054 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0005698"xsd:string |
| http://purl.uniprot.org/citations/19479054 | http://purl.uniprot.org/core/author | "Coles J."xsd:string |
| http://purl.uniprot.org/citations/19479054 | http://purl.uniprot.org/core/author | "Amini F."xsd:string |
| http://purl.uniprot.org/citations/19479054 | http://purl.uniprot.org/core/author | "Zaidi S.H."xsd:string |
| http://purl.uniprot.org/citations/19479054 | http://purl.uniprot.org/core/author | "Bruneau B.G."xsd:string |
| http://purl.uniprot.org/citations/19479054 | http://purl.uniprot.org/core/author | "Takeuchi J.K."xsd:string |
| http://purl.uniprot.org/citations/19479054 | http://purl.uniprot.org/core/author | "Riazi A.M."xsd:string |
| http://purl.uniprot.org/citations/19479054 | http://purl.uniprot.org/core/author | "Hornberger L.K."xsd:string |
| http://purl.uniprot.org/citations/19479054 | http://purl.uniprot.org/core/author | "Van Arsdell G.S."xsd:string |
| http://purl.uniprot.org/citations/19479054 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19479054 | http://purl.uniprot.org/core/name | "PLoS One"xsd:string |
| http://purl.uniprot.org/citations/19479054 | http://purl.uniprot.org/core/pages | "e5698"xsd:string |
| http://purl.uniprot.org/citations/19479054 | http://purl.uniprot.org/core/title | "NKX2-5 regulates the expression of beta-catenin and GATA4 in ventricular myocytes."xsd:string |
| http://purl.uniprot.org/citations/19479054 | http://purl.uniprot.org/core/volume | "4"xsd:string |
| http://purl.uniprot.org/citations/19479054 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19479054 |
| http://purl.uniprot.org/citations/19479054 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19479054 |
| http://purl.uniprot.org/uniprot/P42582#attribution-D810A84D66153E6C17075221A096E8A9 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/19479054 |
| http://purl.uniprot.org/uniprot/P52952#attribution-D415DF7FAA42A287159DCD4D1D55691D | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/19479054 |
| http://purl.uniprot.org/uniprot/#_D3Z7S6-mappedCitation-19479054 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19479054 |
| http://purl.uniprot.org/uniprot/#_D3Z5Q1-mappedCitation-19479054 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19479054 |
| http://purl.uniprot.org/uniprot/#_E9Q6A9-mappedCitation-19479054 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19479054 |
| http://purl.uniprot.org/uniprot/#_P52952-mappedCitation-19479054 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19479054 |
| http://purl.uniprot.org/uniprot/#_P61371-mappedCitation-19479054 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19479054 |