| http://purl.uniprot.org/citations/19481523 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19481523 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19481523 | http://www.w3.org/2000/01/rdf-schema#comment | "H/ACA RNAs form ribonucleoprotein complex (RNP) with proteins Cbf5, Nop10, L7Ae, and Gar1 and guide site-specific conversion of uridine into pseudouridine in cellular RNAs. The crystal structures of H/ACA RNP with substrate bound at the active site cleft reveal that the substrate is recruited through sequence-specific pairing with guide RNA and essential protein contacts. Substrate binding leads to a reorganization of a preset pseudouridylation pocket and an adaptive movement of the PUA domain and the lower stem of the H/ACA RNA. Moreover, a thumb loop flips from the Gar1-bound state in the substrate-free RNP structure to tightly associate with the substrate. Mutagenesis and enzyme kinetics analysis suggest a critical role of Gar1 and the thumb in substrate turnover, particularly in product release. Comparison with tRNA Psi55 synthase TruB reveals the structural conservation and adaptation between an RNA-guided and stand-alone pseudouridine synthase and provides insight into the guide-independent activity of Cbf5."xsd:string |
| http://purl.uniprot.org/citations/19481523 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.molcel.2009.05.005"xsd:string |
| http://purl.uniprot.org/citations/19481523 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.molcel.2009.05.005"xsd:string |
| http://purl.uniprot.org/citations/19481523 | http://purl.uniprot.org/core/author | "Duan J."xsd:string |
| http://purl.uniprot.org/citations/19481523 | http://purl.uniprot.org/core/author | "Duan J."xsd:string |
| http://purl.uniprot.org/citations/19481523 | http://purl.uniprot.org/core/author | "Lu J."xsd:string |
| http://purl.uniprot.org/citations/19481523 | http://purl.uniprot.org/core/author | "Lu J."xsd:string |
| http://purl.uniprot.org/citations/19481523 | http://purl.uniprot.org/core/author | "Li L."xsd:string |
| http://purl.uniprot.org/citations/19481523 | http://purl.uniprot.org/core/author | "Li L."xsd:string |
| http://purl.uniprot.org/citations/19481523 | http://purl.uniprot.org/core/author | "Wang W."xsd:string |
| http://purl.uniprot.org/citations/19481523 | http://purl.uniprot.org/core/author | "Wang W."xsd:string |
| http://purl.uniprot.org/citations/19481523 | http://purl.uniprot.org/core/author | "Ye K."xsd:string |
| http://purl.uniprot.org/citations/19481523 | http://purl.uniprot.org/core/author | "Ye K."xsd:string |
| http://purl.uniprot.org/citations/19481523 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19481523 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19481523 | http://purl.uniprot.org/core/name | "Mol. Cell"xsd:string |
| http://purl.uniprot.org/citations/19481523 | http://purl.uniprot.org/core/name | "Mol Cell"xsd:string |
| http://purl.uniprot.org/citations/19481523 | http://purl.uniprot.org/core/pages | "427-439"xsd:string |
| http://purl.uniprot.org/citations/19481523 | http://purl.uniprot.org/core/pages | "427-439"xsd:string |
| http://purl.uniprot.org/citations/19481523 | http://purl.uniprot.org/core/title | "Structural mechanism of substrate RNA recruitment in H/ACA RNA-guided pseudouridine synthase."xsd:string |
| http://purl.uniprot.org/citations/19481523 | http://purl.uniprot.org/core/title | "Structural mechanism of substrate RNA recruitment in H/ACA RNA-guided pseudouridine synthase."xsd:string |
| http://purl.uniprot.org/citations/19481523 | http://purl.uniprot.org/core/volume | "34"xsd:string |
| http://purl.uniprot.org/citations/19481523 | http://purl.uniprot.org/core/volume | "34"xsd:string |