| http://purl.uniprot.org/citations/19481615 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19481615 | http://www.w3.org/2000/01/rdf-schema#comment | "Lipoxygenases have been classified according to their specificity of fatty acid oxygenation and for several plant enzymes pH-dependent alterations in the product patterns have been reported. Assuming that the biological role of mammalian lipoxygenases is based on the formation of specific reaction products, pH-dependent alterations would impact enzymes' functionality. In this study we systematically investigated the pH-dependence of vertebrate lipoxygenases and observed a remarkable stability of the product pattern in the near physiological range for the wild-type enzyme species. Site-directed mutagenesis of selected amino acids and alterations in the substrate concentrations induced a more pronounced pH-dependence of the reaction specificity. For instance, for the V603H mutant of the human 15-lipoxygenase-2 8-lipoxygenation was dominant at acidic pH (65%) whereas 15-H(p)ETE was the major oxygenation product at pH 8. Similarly, the product pattern of the wild-type mouse 8-lipoxygenase was hardly altered in the near physiological pH range but H604F exchange induced strong pH-dependent alterations in the positional specificity. Taken together, our data suggest that the reaction specificities of wild-type vertebrate lipoxygenase isoforms are largely resistant towards pH alterations. However, we found that changes in the assay conditions (low substrate concentration) and introduction/removal of a critical histidine at the active site impact the pH-dependence of reaction specificity for some lipoxygenase isoforms."xsd:string |
| http://purl.uniprot.org/citations/19481615 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbalip.2009.05.007"xsd:string |
| http://purl.uniprot.org/citations/19481615 | http://purl.uniprot.org/core/author | "Kuhn H."xsd:string |
| http://purl.uniprot.org/citations/19481615 | http://purl.uniprot.org/core/author | "Walther M."xsd:string |
| http://purl.uniprot.org/citations/19481615 | http://purl.uniprot.org/core/author | "Jansen C."xsd:string |
| http://purl.uniprot.org/citations/19481615 | http://purl.uniprot.org/core/author | "Ivanov I."xsd:string |
| http://purl.uniprot.org/citations/19481615 | http://purl.uniprot.org/core/author | "Anton M."xsd:string |
| http://purl.uniprot.org/citations/19481615 | http://purl.uniprot.org/core/author | "Roffeis J."xsd:string |
| http://purl.uniprot.org/citations/19481615 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19481615 | http://purl.uniprot.org/core/name | "Biochim Biophys Acta"xsd:string |
| http://purl.uniprot.org/citations/19481615 | http://purl.uniprot.org/core/pages | "827-835"xsd:string |
| http://purl.uniprot.org/citations/19481615 | http://purl.uniprot.org/core/title | "Structural basis for pH-dependent alterations of reaction specificity of vertebrate lipoxygenase isoforms."xsd:string |
| http://purl.uniprot.org/citations/19481615 | http://purl.uniprot.org/core/volume | "1791"xsd:string |
| http://purl.uniprot.org/citations/19481615 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19481615 |
| http://purl.uniprot.org/citations/19481615 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19481615 |
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