Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/19492014http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/19492014http://www.w3.org/2000/01/rdf-schema#comment"

Background

Voltage-gated Ca(v)1.2 calcium channels play a crucial role in Ca(2+) signaling. The pore-forming alpha(1C) subunit is regulated by accessory Ca(v)beta subunits, cytoplasmic proteins of various size encoded by four different genes (Ca(v)beta(1)-beta(4)) and expressed in a tissue-specific manner.

Methods and results

Here we investigated the effect of three major Ca(v)beta types, beta(1b), beta(2d) and beta(3), on the structure of Ca(v)1.2 in the plasma membrane of live cells. Total internal reflection fluorescence microscopy showed that the tendency of Ca(v)1.2 to form clusters depends on the type of the Ca(v)beta subunit present. The highest density of Ca(v)1.2 clusters in the plasma membrane and the smallest cluster size were observed with neuronal/cardiac beta(1b) present. Ca(v)1.2 channels containing beta(3), the predominant Ca(v)beta subunit of vascular smooth muscle cells, were organized in a significantly smaller number of larger clusters. The inter- and intramolecular distances between alpha(1C) and Ca(v)beta in the plasma membrane of live cells were measured by three-color FRET microscopy. The results confirm that the proximity of Ca(v)1.2 channels in the plasma membrane depends on the Ca(v)beta type. The presence of different Ca(v)beta subunits does not result in significant differences in the intramolecular distance between the termini of alpha(1C), but significantly affects the distance between the termini of neighbor alpha(1C) subunits, which varies from 67 A with beta(1b) to 79 A with beta(3).

Conclusions

Thus, our results show that the structural organization of Ca(v)1.2 channels in the plasma membrane depends on the type of Ca(v)beta subunits present."xsd:string
http://purl.uniprot.org/citations/19492014http://purl.org/dc/terms/identifier"doi:10.1371/journal.pone.0005587"xsd:string
http://purl.uniprot.org/citations/19492014http://purl.uniprot.org/core/author"Thomas S."xsd:string
http://purl.uniprot.org/citations/19492014http://purl.uniprot.org/core/author"Patel C."xsd:string
http://purl.uniprot.org/citations/19492014http://purl.uniprot.org/core/author"Harry J.B."xsd:string
http://purl.uniprot.org/citations/19492014http://purl.uniprot.org/core/author"Kobrinsky E."xsd:string
http://purl.uniprot.org/citations/19492014http://purl.uniprot.org/core/author"Soldatov N.M."xsd:string
http://purl.uniprot.org/citations/19492014http://purl.uniprot.org/core/author"Duong S.Q."xsd:string
http://purl.uniprot.org/citations/19492014http://purl.uniprot.org/core/author"Lao Q.Z."xsd:string
http://purl.uniprot.org/citations/19492014http://purl.uniprot.org/core/author"Abrahimi P."xsd:string
http://purl.uniprot.org/citations/19492014http://purl.uniprot.org/core/date"2009"xsd:gYear
http://purl.uniprot.org/citations/19492014http://purl.uniprot.org/core/name"PLoS One"xsd:string
http://purl.uniprot.org/citations/19492014http://purl.uniprot.org/core/pages"e5587"xsd:string
http://purl.uniprot.org/citations/19492014http://purl.uniprot.org/core/title"Effect of Ca(v)beta subunits on structural organization of Ca(v)1.2 calcium channels."xsd:string
http://purl.uniprot.org/citations/19492014http://purl.uniprot.org/core/volume"4"xsd:string
http://purl.uniprot.org/citations/19492014http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/19492014
http://purl.uniprot.org/citations/19492014http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/19492014
http://purl.uniprot.org/uniprot/#_A0A0A0MR67-mappedCitation-19492014http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19492014
http://purl.uniprot.org/uniprot/#_A0A0A0MSA1-mappedCitation-19492014http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19492014
http://purl.uniprot.org/uniprot/#_E9PDI6-mappedCitation-19492014http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19492014
http://purl.uniprot.org/uniprot/#_A0A804HI37-mappedCitation-19492014http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19492014
http://purl.uniprot.org/uniprot/#_A0A804HIJ8-mappedCitation-19492014http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19492014
http://purl.uniprot.org/uniprot/#_A0A804HJB6-mappedCitation-19492014http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19492014
http://purl.uniprot.org/uniprot/#_A0A804HKC4-mappedCitation-19492014http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19492014
▶