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http://purl.uniprot.org/citations/19520148http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/19520148http://www.w3.org/2000/01/rdf-schema#comment"

Background

The determination of protein-protein interfaces is of crucial importance to understand protein function and to guide the design of compounds. To identify protein-protein interface by NMR spectroscopy, 13C NMR paramagnetic shifts induced by freely diffusing 4-hydroxy-2, 2, 6, 6-tetramethyl-piperidine-1-oxyl (TEMPOL) are promising, because TEMPOL affects distinct 13C NMR chemical shifts of the solvent accessible nuclei belonging to proteins of interest, while 13C nuclei within the interior of the proteins may be distinguished by a lack of such shifts.

Method

We measured the 13C NMR paramagnetic shifts induced by TEMPOL by recording 13C-(13)C TOCSY spectra for ubiquitin in the free state and the complex state with yeast ubiquitin hydrolase1 (YUH1).

Results

Upon complexation of ubiquitin with YUH1, 13C NMR paramagnetic shifts associated with the protein binding interface were reduced by 0.05 ppm or more. The identified interfacial atoms agreed with the prior X-ray crystallographic data.

Conclusions

The TEMPOL-induced 13C chemical shift perturbation is useful to determine precise protein-protein interfaces.

General significance

The present method is a useful method to determine protein-protein interface by NMR, because it has advantages in easy sample preparations, simple data analyses, and wide applicabilities."xsd:string
http://purl.uniprot.org/citations/19520148http://purl.org/dc/terms/identifier"doi:10.1016/j.bbagen.2009.06.001"xsd:string
http://purl.uniprot.org/citations/19520148http://purl.uniprot.org/core/author"Shimada I."xsd:string
http://purl.uniprot.org/citations/19520148http://purl.uniprot.org/core/author"Sakakura M."xsd:string
http://purl.uniprot.org/citations/19520148http://purl.uniprot.org/core/author"Tokunaga Y."xsd:string
http://purl.uniprot.org/citations/19520148http://purl.uniprot.org/core/author"Moriya J."xsd:string
http://purl.uniprot.org/citations/19520148http://purl.uniprot.org/core/author"Prosser R.S."xsd:string
http://purl.uniprot.org/citations/19520148http://purl.uniprot.org/core/date"2009"xsd:gYear
http://purl.uniprot.org/citations/19520148http://purl.uniprot.org/core/name"Biochim Biophys Acta"xsd:string
http://purl.uniprot.org/citations/19520148http://purl.uniprot.org/core/pages"1368-1376"xsd:string
http://purl.uniprot.org/citations/19520148http://purl.uniprot.org/core/title"An NMR method for the determination of protein binding interfaces using TEMPOL-induced chemical shift perturbations."xsd:string
http://purl.uniprot.org/citations/19520148http://purl.uniprot.org/core/volume"1790"xsd:string
http://purl.uniprot.org/citations/19520148http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/19520148
http://purl.uniprot.org/citations/19520148http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/19520148
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