| http://purl.uniprot.org/citations/19528539 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19528539 | http://www.w3.org/2000/01/rdf-schema#comment | "The small guanine-nucleotide-binding protein Rap1 plays a key role in platelet aggregation and hemostasis, and we recently identified Rap1GAP2 as the only GTPase-activating protein of Rap1 in platelets. In search of Rap1GAP2-associated proteins, we performed yeast-2-hybrid screening and found synaptotagmin-like protein 1 (Slp1) as a new binding partner. We confirmed the interaction of Rap1GAP2 and Slp1 in transfected COS-1 and HeLa cells and at endogenous level in human platelets. Mapping studies showed that Rap1GAP2 binds through amino acids T524-K525-X-T527 within its C-terminus to the C2A domain of Slp1. Slp1 contains a Rab27-binding domain, and we demonstrate that Rap1GAP2, Slp1, and Rab27 form a trimeric complex in transfected cells and in platelets. Purified Slp1 dose-dependently decreased dense granule secretion in streptolysin-O-permeabilized platelets stimulated with calcium or guanosine 5'-O-[gamma-thio] triphosphate. The isolated C2A domain of Slp1 had a stimulatory effect on granule secretion and reversed the inhibitory effect of full-length Slp1. Purified Rap1GAP2 augmented dense granule secretion of permeabilized platelets, whereas deletion of the Slp1-binding TKXT motif abolished the effect of Rap1GAP2. We conclude that Slp1 inhibits dense granule secretion in platelets and that Rap1GAP2 modulates secretion by binding to Slp1."xsd:string |
| http://purl.uniprot.org/citations/19528539 | http://purl.org/dc/terms/identifier | "doi:10.1182/blood-2008-05-155234"xsd:string |
| http://purl.uniprot.org/citations/19528539 | http://purl.uniprot.org/core/author | "Hoffmeister M."xsd:string |
| http://purl.uniprot.org/citations/19528539 | http://purl.uniprot.org/core/author | "Prelle C."xsd:string |
| http://purl.uniprot.org/citations/19528539 | http://purl.uniprot.org/core/author | "Smolenski A.P."xsd:string |
| http://purl.uniprot.org/citations/19528539 | http://purl.uniprot.org/core/author | "Neumuller O."xsd:string |
| http://purl.uniprot.org/citations/19528539 | http://purl.uniprot.org/core/author | "Gegenbauer K."xsd:string |
| http://purl.uniprot.org/citations/19528539 | http://purl.uniprot.org/core/author | "Babica J."xsd:string |
| http://purl.uniprot.org/citations/19528539 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19528539 | http://purl.uniprot.org/core/name | "Blood"xsd:string |
| http://purl.uniprot.org/citations/19528539 | http://purl.uniprot.org/core/pages | "1396-1404"xsd:string |
| http://purl.uniprot.org/citations/19528539 | http://purl.uniprot.org/core/title | "Synaptotagmin-like protein 1 interacts with the GTPase-activating protein Rap1GAP2 and regulates dense granule secretion in platelets."xsd:string |
| http://purl.uniprot.org/citations/19528539 | http://purl.uniprot.org/core/volume | "114"xsd:string |
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