| http://purl.uniprot.org/citations/1952936 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
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| http://purl.uniprot.org/citations/1952936 | http://www.w3.org/2000/01/rdf-schema#comment | "The NAD-dependent glutamate dehydrogenase (GDH) from Dictyostelium discoideum was purified 1101-fold with a yield of 23.4%. The enzyme has an apparent Mr of 356 kDa, determined using Sephacryl S400, and a subunit molecular weight of 54 kDa on SDS-polyacrylamide gel electrophoresis. The Kms for alpha-ketoglutarate, NADH, and NH4+ are 0.36 +/- 0.03 mM, 16.0 +/- 0.1 microM, and 34.5 +/-2.7 mM, respectively. The purified enzyme has a pH optimum of pH 7.25-7.5. At 0.1 mM, ADP and AMP stimulate GDH activity 25 and 102%, respectively. Half-maximal activity in the presence of 0.1 mM AMP for alpha-ketoglutarate, NADH, and NH4+ is reached at 2.3 +/- 0.1 mM, 71.4 +/- 5.5 microM, and 27.9 +/-3.6 mM, respectively."xsd:string |
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| http://purl.uniprot.org/citations/1952936 | http://purl.uniprot.org/core/author | "Pamula F."xsd:string |
| http://purl.uniprot.org/citations/1952936 | http://purl.uniprot.org/core/author | "Wheldrake J.F."xsd:string |
| http://purl.uniprot.org/citations/1952936 | http://purl.uniprot.org/core/author | "Wheldrake J.F."xsd:string |
| http://purl.uniprot.org/citations/1952936 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
| http://purl.uniprot.org/citations/1952936 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
| http://purl.uniprot.org/citations/1952936 | http://purl.uniprot.org/core/name | "Arch. Biochem. Biophys."xsd:string |
| http://purl.uniprot.org/citations/1952936 | http://purl.uniprot.org/core/name | "Arch. Biochem. Biophys."xsd:string |
| http://purl.uniprot.org/citations/1952936 | http://purl.uniprot.org/core/pages | "225-230"xsd:string |
| http://purl.uniprot.org/citations/1952936 | http://purl.uniprot.org/core/pages | "225-230"xsd:string |
| http://purl.uniprot.org/citations/1952936 | http://purl.uniprot.org/core/title | "The NAD-dependent glutamate dehydrogenase from Dictyostelium discoideum: purification and properties."xsd:string |
| http://purl.uniprot.org/citations/1952936 | http://purl.uniprot.org/core/title | "The NAD-dependent glutamate dehydrogenase from Dictyostelium discoideum: purification and properties."xsd:string |
| http://purl.uniprot.org/citations/1952936 | http://purl.uniprot.org/core/volume | "291"xsd:string |
| http://purl.uniprot.org/citations/1952936 | http://purl.uniprot.org/core/volume | "291"xsd:string |
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| http://purl.uniprot.org/citations/1952936 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1952936 |
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| http://purl.uniprot.org/uniprot/Q54KB7 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/1952936 |