| http://purl.uniprot.org/citations/19540419 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19540419 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19540419 | http://www.w3.org/2000/01/rdf-schema#comment | "Jug r 1, the 2S albumin allergen from walnut, was isolated from ripe nuts as a native allergen and expressed in Escherichia coli using the Gateway technology as a recombinant allergen. The recombinant Jug r 1 (15 kDa) differs from the native allergen by the absence of cleavage of the polypeptide chain in two covalently associated light (3.5 kDa) and heavy (8 kDa) chains. Recombinant rJug r 1 adopts the canonical alpha-helical fold of plant 2S albumins as checked on CD spectra. Four IgE-binding epitopic stretches were identified along the amino acid sequence of Jug r 1 and localized on the molecular surface of the modeled allergen. Both native and recombinant allergens exhibit similar IgE-binding activity and similarly trigger the degranulation of a FcepsilonRI-expressing rat basophilic leukaemia cell line previously treated by IgE-containing sera. Native Jug r 1 resists to heat denaturation and to the proteolytic attack of trypsin and chymotrypsin but is readily hydrolyzed in the presence of pepsin at acidic pH after 1 h of incubation at 37 degrees C in vitro. Recombinant Jug r 1 could be used for a component-resolved diagnosis of food-allergy."xsd:string |
| http://purl.uniprot.org/citations/19540419 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.peptides.2009.03.007"xsd:string |
| http://purl.uniprot.org/citations/19540419 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.peptides.2009.03.007"xsd:string |
| http://purl.uniprot.org/citations/19540419 | http://purl.uniprot.org/core/author | "Barre A."xsd:string |
| http://purl.uniprot.org/citations/19540419 | http://purl.uniprot.org/core/author | "Barre A."xsd:string |
| http://purl.uniprot.org/citations/19540419 | http://purl.uniprot.org/core/author | "Culerrier R."xsd:string |
| http://purl.uniprot.org/citations/19540419 | http://purl.uniprot.org/core/author | "Culerrier R."xsd:string |
| http://purl.uniprot.org/citations/19540419 | http://purl.uniprot.org/core/author | "Didier A."xsd:string |
| http://purl.uniprot.org/citations/19540419 | http://purl.uniprot.org/core/author | "Didier A."xsd:string |
| http://purl.uniprot.org/citations/19540419 | http://purl.uniprot.org/core/author | "Granier C."xsd:string |
| http://purl.uniprot.org/citations/19540419 | http://purl.uniprot.org/core/author | "Granier C."xsd:string |
| http://purl.uniprot.org/citations/19540419 | http://purl.uniprot.org/core/author | "Rance F."xsd:string |
| http://purl.uniprot.org/citations/19540419 | http://purl.uniprot.org/core/author | "Rance F."xsd:string |
| http://purl.uniprot.org/citations/19540419 | http://purl.uniprot.org/core/author | "Rouge P."xsd:string |
| http://purl.uniprot.org/citations/19540419 | http://purl.uniprot.org/core/author | "Rouge P."xsd:string |
| http://purl.uniprot.org/citations/19540419 | http://purl.uniprot.org/core/author | "Sordet C."xsd:string |
| http://purl.uniprot.org/citations/19540419 | http://purl.uniprot.org/core/author | "Sordet C."xsd:string |
| http://purl.uniprot.org/citations/19540419 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19540419 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19540419 | http://purl.uniprot.org/core/name | "Peptides"xsd:string |
| http://purl.uniprot.org/citations/19540419 | http://purl.uniprot.org/core/name | "Peptides"xsd:string |
| http://purl.uniprot.org/citations/19540419 | http://purl.uniprot.org/core/pages | "1213-1221"xsd:string |
| http://purl.uniprot.org/citations/19540419 | http://purl.uniprot.org/core/pages | "1213-1221"xsd:string |