| http://purl.uniprot.org/citations/19541769 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19541769 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19541769 | http://www.w3.org/2000/01/rdf-schema#comment | "The positive-strand RNA genome of the Hepatitis C virus (HCV) contains an internal ribosome entry site (IRES) in the 5'untranslated region (5'UTR) and structured sequence elements within the 3'UTR, but no poly(A) tail. Employing a limited set of initiation factors, the HCV IRES coordinates the 5'cap-independent assembly of the 43S pre-initiation complex at an internal initiation codon located in the IRES sequence. We have established a Huh7 cell-derived in vitro translation system that shows a 3'UTR-dependent enhancement of 43S pre-initiation complex formation at the HCV IRES. Through the use of tobramycin (Tob)-aptamer affinity chromatography, we identified the Insulin-like growth factor-II mRNA-binding protein 1 (IGF2BP1) as a factor that interacts with both, the HCV 5'UTR and 3'UTR. We report that IGF2BP1 specifically enhances translation at the HCV IRES, but it does not affect 5'cap-dependent translation. RNA interference against IGF2BP1 in HCV replicon RNA-containing Huh7 cells reduces HCV IRES-mediated translation, whereas replication remains unaffected. Interestingly, we found that endogenous IGF2BP1 specifically co-immunoprecipitates with HCV replicon RNA, the ribosomal 40S subunit, and eIF3. Furthermore eIF3 comigrates with IGF2BP1 in 80S ribosomal complexes when a reporter mRNA bearing both the HCV 5'UTR and HCV 3'UTR is translated. Our data suggest that IGF2BP1, by binding to the HCV 5'UTR and/or HCV 3'UTR, recruits eIF3 and enhances HCV IRES-mediated translation."xsd:string |
| http://purl.uniprot.org/citations/19541769 | http://purl.org/dc/terms/identifier | "doi:10.1261/rna.1578409"xsd:string |
| http://purl.uniprot.org/citations/19541769 | http://purl.org/dc/terms/identifier | "doi:10.1261/rna.1578409"xsd:string |
| http://purl.uniprot.org/citations/19541769 | http://purl.uniprot.org/core/author | "Huettelmaier S."xsd:string |
| http://purl.uniprot.org/citations/19541769 | http://purl.uniprot.org/core/author | "Huettelmaier S."xsd:string |
| http://purl.uniprot.org/citations/19541769 | http://purl.uniprot.org/core/author | "Schierhorn A."xsd:string |
| http://purl.uniprot.org/citations/19541769 | http://purl.uniprot.org/core/author | "Schierhorn A."xsd:string |
| http://purl.uniprot.org/citations/19541769 | http://purl.uniprot.org/core/author | "Ostareck-Lederer A."xsd:string |
| http://purl.uniprot.org/citations/19541769 | http://purl.uniprot.org/core/author | "Ostareck-Lederer A."xsd:string |
| http://purl.uniprot.org/citations/19541769 | http://purl.uniprot.org/core/author | "Behrens S.-E."xsd:string |
| http://purl.uniprot.org/citations/19541769 | http://purl.uniprot.org/core/author | "Behrens S.-E."xsd:string |
| http://purl.uniprot.org/citations/19541769 | http://purl.uniprot.org/core/author | "Ostareck D.H."xsd:string |
| http://purl.uniprot.org/citations/19541769 | http://purl.uniprot.org/core/author | "Ostareck D.H."xsd:string |
| http://purl.uniprot.org/citations/19541769 | http://purl.uniprot.org/core/author | "Weinlich S."xsd:string |
| http://purl.uniprot.org/citations/19541769 | http://purl.uniprot.org/core/author | "Weinlich S."xsd:string |
| http://purl.uniprot.org/citations/19541769 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19541769 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19541769 | http://purl.uniprot.org/core/name | "RNA"xsd:string |
| http://purl.uniprot.org/citations/19541769 | http://purl.uniprot.org/core/name | "RNA"xsd:string |
| http://purl.uniprot.org/citations/19541769 | http://purl.uniprot.org/core/pages | "1528-1542"xsd:string |
| http://purl.uniprot.org/citations/19541769 | http://purl.uniprot.org/core/pages | "1528-1542"xsd:string |
| http://purl.uniprot.org/citations/19541769 | http://purl.uniprot.org/core/title | "IGF2BP1 enhances HCV IRES-mediated translation initiation via the 3'UTR."xsd:string |
| http://purl.uniprot.org/citations/19541769 | http://purl.uniprot.org/core/title | "IGF2BP1 enhances HCV IRES-mediated translation initiation via the 3'UTR."xsd:string |