| http://purl.uniprot.org/citations/19542234 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19542234 | http://www.w3.org/2000/01/rdf-schema#comment | "Despite extensive characterization of the mu-opioid receptor (MOR), the biochemical properties of the isolated receptor remain unclear. In light of recent reports, we proposed that the monomeric form of MOR can activate G proteins and be subject to allosteric regulation. A mu-opioid receptor fused to yellow fluorescent protein (YMOR) was constructed and expressed in insect cells. YMOR binds ligands with high affinity, displays agonist-stimulated [(35)S]guanosine 5'-(gamma-thio)triphosphate binding to Galpha(i), and is allosterically regulated by coupled G(i) protein heterotrimer both in insect cell membranes and as purified protein reconstituted into a phospholipid bilayer in the form of high density lipoprotein particles. Single-particle imaging of fluorescently labeled receptor indicates that the reconstituted YMOR is monomeric. Moreover, single-molecule imaging of a Cy3-labeled agonist, [Lys(7), Cys(8)]dermorphin, illustrates a novel method for studying G protein-coupled receptor-ligand binding and suggests that one molecule of agonist binds per monomeric YMOR. Together these data support the notion that oligomerization of the mu-opioid receptor is not required for agonist and antagonist binding and that the monomeric receptor is the minimal functional unit in regard to G protein activation and strong allosteric regulation of agonist binding by G proteins."xsd:string |
| http://purl.uniprot.org/citations/19542234 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m109.026922"xsd:string |
| http://purl.uniprot.org/citations/19542234 | http://purl.uniprot.org/core/author | "Sunahara R.K."xsd:string |
| http://purl.uniprot.org/citations/19542234 | http://purl.uniprot.org/core/author | "Mosberg H.I."xsd:string |
| http://purl.uniprot.org/citations/19542234 | http://purl.uniprot.org/core/author | "Walter N.G."xsd:string |
| http://purl.uniprot.org/citations/19542234 | http://purl.uniprot.org/core/author | "Kuszak A.J."xsd:string |
| http://purl.uniprot.org/citations/19542234 | http://purl.uniprot.org/core/author | "Pitchiaya S."xsd:string |
| http://purl.uniprot.org/citations/19542234 | http://purl.uniprot.org/core/author | "Anand J.P."xsd:string |
| http://purl.uniprot.org/citations/19542234 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19542234 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/19542234 | http://purl.uniprot.org/core/pages | "26732-26741"xsd:string |
| http://purl.uniprot.org/citations/19542234 | http://purl.uniprot.org/core/title | "Purification and functional reconstitution of monomeric mu-opioid receptors: allosteric modulation of agonist binding by Gi2."xsd:string |
| http://purl.uniprot.org/citations/19542234 | http://purl.uniprot.org/core/volume | "284"xsd:string |
| http://purl.uniprot.org/citations/19542234 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19542234 |
| http://purl.uniprot.org/citations/19542234 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19542234 |
| http://purl.uniprot.org/uniprot/#_A0A0F6TN24-mappedCitation-19542234 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19542234 |
| http://purl.uniprot.org/uniprot/#_A0A089PQW6-mappedCitation-19542234 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19542234 |
| http://purl.uniprot.org/uniprot/#_A0A0F6TMB4-mappedCitation-19542234 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19542234 |
| http://purl.uniprot.org/uniprot/#_A0A0F6TMM4-mappedCitation-19542234 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19542234 |
| http://purl.uniprot.org/uniprot/#_A0A0F6TMT4-mappedCitation-19542234 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19542234 |
| http://purl.uniprot.org/uniprot/#_A0A0F6RAI2-mappedCitation-19542234 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19542234 |
| http://purl.uniprot.org/uniprot/#_A0A0F6RA07-mappedCitation-19542234 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19542234 |
| http://purl.uniprot.org/uniprot/#_A0A6H0WCP3-mappedCitation-19542234 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19542234 |
| http://purl.uniprot.org/uniprot/#_A0A6H0WF09-mappedCitation-19542234 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19542234 |