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http://purl.uniprot.org/citations/19542345http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/19542345http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/19542345http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/19542345http://www.w3.org/2000/01/rdf-schema#comment"The whole-genome sequence of the endosymbiotic bacterium Azorhizobium caulinodans ORS571, which forms nitrogen-fixing nodules on the stems and roots of Sesbania rostrata, was recently determined. The sizes of the genome and symbiosis island are 5.4 Mb and 86.7 kb, respectively, and these sizes are the smallest among the sequenced rhizobia. In the present study, a whole-genome microarray of A. caulinodans was constructed, and transcriptomic analyses were performed on free-living cells grown in rich and minimal media and in bacteroids isolated from stem nodules. Transcriptional profiling showed that the genes involved in sulfur uptake and metabolism, acetone metabolism, and the biosynthesis of exopolysaccharide were highly expressed in bacteroids compared to the expression levels in free-living cells. Some mutants having Tn5 transposons within these genes with increased expression were obtained as nodule-deficient mutants in our previous study. A transcriptomic analysis was also performed on free-living cells grown in minimal medium supplemented with a flavonoid, naringenin, which is one of the most efficient inducers of A. caulinodans nod genes. Only 18 genes exhibited increased expression by the addition of naringenin, suggesting that the regulatory mechanism responding to the flavonoid could be simple in A. caulinodans. The combination of our genome-wide transcriptional profiling and our previous genome-wide mutagenesis study has revealed new aspects of nodule formation and maintenance."xsd:string
http://purl.uniprot.org/citations/19542345http://purl.org/dc/terms/identifier"doi:10.1128/AEM.00398-09"xsd:string
http://purl.uniprot.org/citations/19542345http://purl.org/dc/terms/identifier"doi:10.1128/aem.00398-09"xsd:string
http://purl.uniprot.org/citations/19542345http://purl.org/dc/terms/identifier"doi:10.1128/aem.00398-09"xsd:string
http://purl.uniprot.org/citations/19542345http://purl.uniprot.org/core/author"Tsukada S."xsd:string
http://purl.uniprot.org/citations/19542345http://purl.uniprot.org/core/author"Tsukada S."xsd:string
http://purl.uniprot.org/citations/19542345http://purl.uniprot.org/core/author"Tsukada S."xsd:string
http://purl.uniprot.org/citations/19542345http://purl.uniprot.org/core/author"Oyaizu H."xsd:string
http://purl.uniprot.org/citations/19542345http://purl.uniprot.org/core/author"Oyaizu H."xsd:string
http://purl.uniprot.org/citations/19542345http://purl.uniprot.org/core/author"Oyaizu H."xsd:string
http://purl.uniprot.org/citations/19542345http://purl.uniprot.org/core/author"Aono T."xsd:string
http://purl.uniprot.org/citations/19542345http://purl.uniprot.org/core/author"Aono T."xsd:string
http://purl.uniprot.org/citations/19542345http://purl.uniprot.org/core/author"Aono T."xsd:string
http://purl.uniprot.org/citations/19542345http://purl.uniprot.org/core/author"Lee K.B."xsd:string
http://purl.uniprot.org/citations/19542345http://purl.uniprot.org/core/author"Liu C.T."xsd:string
http://purl.uniprot.org/citations/19542345http://purl.uniprot.org/core/author"Akiba N."xsd:string
http://purl.uniprot.org/citations/19542345http://purl.uniprot.org/core/author"Akiba N."xsd:string
http://purl.uniprot.org/citations/19542345http://purl.uniprot.org/core/author"Akiba N."xsd:string
http://purl.uniprot.org/citations/19542345http://purl.uniprot.org/core/author"Lee KB."xsd:string
http://purl.uniprot.org/citations/19542345http://purl.uniprot.org/core/author"Lee KB."xsd:string
http://purl.uniprot.org/citations/19542345http://purl.uniprot.org/core/author"Liu CT."xsd:string
http://purl.uniprot.org/citations/19542345http://purl.uniprot.org/core/author"Liu CT."xsd:string