| http://purl.uniprot.org/citations/19561594 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19561594 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19561594 | http://www.w3.org/2000/01/rdf-schema#comment | "Metazoan genomes encode hundreds of RNA-binding proteins (RBPs) but RNA-binding preferences for relatively few RBPs have been well defined. Current techniques for determining RNA targets, including in vitro selection and RNA co-immunoprecipitation, require significant time and labor investment. Here we introduce RNAcompete, a method for the systematic analysis of RNA binding specificities that uses a single binding reaction to determine the relative preferences of RBPs for short RNAs that contain a complete range of k-mers in structured and unstructured RNA contexts. We tested RNAcompete by analyzing nine diverse RBPs (HuR, Vts1, FUSIP1, PTB, U1A, SF2/ASF, SLM2, RBM4 and YB1). RNAcompete identified expected and previously unknown RNA binding preferences. Using in vitro and in vivo binding data, we demonstrate that preferences for individual 7-mers identified by RNAcompete are a more accurate representation of binding activity than are conventional motif models. We anticipate that RNAcompete will be a valuable tool for the study of RNA-protein interactions."xsd:string |
| http://purl.uniprot.org/citations/19561594 | http://purl.org/dc/terms/identifier | "doi:10.1038/nbt.1550"xsd:string |
| http://purl.uniprot.org/citations/19561594 | http://purl.org/dc/terms/identifier | "doi:10.1038/nbt.1550"xsd:string |
| http://purl.uniprot.org/citations/19561594 | http://purl.uniprot.org/core/author | "Chaudhry S."xsd:string |
| http://purl.uniprot.org/citations/19561594 | http://purl.uniprot.org/core/author | "Chaudhry S."xsd:string |
| http://purl.uniprot.org/citations/19561594 | http://purl.uniprot.org/core/author | "Hughes T.R."xsd:string |
| http://purl.uniprot.org/citations/19561594 | http://purl.uniprot.org/core/author | "Hughes T.R."xsd:string |
| http://purl.uniprot.org/citations/19561594 | http://purl.uniprot.org/core/author | "Morris Q."xsd:string |
| http://purl.uniprot.org/citations/19561594 | http://purl.uniprot.org/core/author | "Morris Q."xsd:string |
| http://purl.uniprot.org/citations/19561594 | http://purl.uniprot.org/core/author | "Blencowe B.J."xsd:string |
| http://purl.uniprot.org/citations/19561594 | http://purl.uniprot.org/core/author | "Blencowe B.J."xsd:string |
| http://purl.uniprot.org/citations/19561594 | http://purl.uniprot.org/core/author | "Ray D."xsd:string |
| http://purl.uniprot.org/citations/19561594 | http://purl.uniprot.org/core/author | "Ray D."xsd:string |
| http://purl.uniprot.org/citations/19561594 | http://purl.uniprot.org/core/author | "Chan E.T."xsd:string |
| http://purl.uniprot.org/citations/19561594 | http://purl.uniprot.org/core/author | "Chan E.T."xsd:string |
| http://purl.uniprot.org/citations/19561594 | http://purl.uniprot.org/core/author | "Talukder S."xsd:string |
| http://purl.uniprot.org/citations/19561594 | http://purl.uniprot.org/core/author | "Talukder S."xsd:string |
| http://purl.uniprot.org/citations/19561594 | http://purl.uniprot.org/core/author | "Pena Castillo L."xsd:string |
| http://purl.uniprot.org/citations/19561594 | http://purl.uniprot.org/core/author | "Pena Castillo L."xsd:string |
| http://purl.uniprot.org/citations/19561594 | http://purl.uniprot.org/core/author | "Kazan H."xsd:string |
| http://purl.uniprot.org/citations/19561594 | http://purl.uniprot.org/core/author | "Kazan H."xsd:string |
| http://purl.uniprot.org/citations/19561594 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19561594 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |