Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/19619438http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/19619438http://www.w3.org/2000/01/rdf-schema#comment"

Background and objective

Inducing gene demethylation may be a mechanism of arsenic trioxide (As(2)O(3)) in treating hematologic cancers. This study was to investigate the effect of As(2)O(3) on demethylation of SH2-containing phosphatase-1 (SHP-1) in human lymphoma cell line T2 and on the proliferation of T2 cells.

Methods

T2 cells were treated with As(2)O(3) and 5-aza-2'-deoxyoytidine (5-AC) alone or in combination. The methylation of SHP-1 in T2 cells was detected by methylation-specific polymerase chain reaction (MSP). The mRNA and protein expression of SHP-1 were determined by fluorescence quantitative-polymerase chain reaction (FQ-PCR) and Western blot. The expression of p-c-kit was detected by Western blot. Cell proliferation was detected by MTT assay. Cell apoptosis was detected by flow cytometry.

Results

As(2)O(3) led to progressive demethylation and re-expression of SHP-1 in T2 cells, as well as down-regulation of phosphorylated c-kit. As(2)O(3) inhibited the proliferation and promoted the apoptosis of T2 cells, and its effects were enhanced along with the increase of concentration and treatment time (p<0.05). The proliferation inhibition rates were significantly lower in As(2)O(3) (2.5 micromol/L) group than in combination (2.5 micromol/L As(2)O(3) plus 2 micromol/L 5-AC) group (9.8% vs. 11.0%on Day 1, 20.3% vs. 36.7% on Day 2, 47.5% vs. 61.0% on Day 3, p<0.05). The apoptosis rates were significantly higher in combination group than in As(2)O(3) group (17.3% vs. 6.1% on Day 1, 37.9% vs. 26.5% on Day 2, 67.9% vs. 50.9% on Day 3, p<0.05).

Conclusions

As(2)O(3) could cause demethylation and re-expression of SHP-1 in T2 cells, and may inhibit proliferation and induce apoptosis of T2 cells via suppressing activation of c-kit receptor and its signal transduction."xsd:string
http://purl.uniprot.org/citations/19619438http://purl.uniprot.org/core/author"Li Y."xsd:string
http://purl.uniprot.org/citations/19619438http://purl.uniprot.org/core/author"Yang L."xsd:string
http://purl.uniprot.org/citations/19619438http://purl.uniprot.org/core/author"Liu X.J."xsd:string
http://purl.uniprot.org/citations/19619438http://purl.uniprot.org/core/author"Du X.Y."xsd:string
http://purl.uniprot.org/citations/19619438http://purl.uniprot.org/core/author"Wen S.P."xsd:string
http://purl.uniprot.org/citations/19619438http://purl.uniprot.org/core/author"Luo J.M."xsd:string
http://purl.uniprot.org/citations/19619438http://purl.uniprot.org/core/date"2009"xsd:gYear
http://purl.uniprot.org/citations/19619438http://purl.uniprot.org/core/name"Ai Zheng"xsd:string
http://purl.uniprot.org/citations/19619438http://purl.uniprot.org/core/pages"249-254"xsd:string
http://purl.uniprot.org/citations/19619438http://purl.uniprot.org/core/title"[Effect of As2O3 on demethylation of SHP-1 gene in human lymphoma cell line T2]."xsd:string
http://purl.uniprot.org/citations/19619438http://purl.uniprot.org/core/volume"28"xsd:string
http://purl.uniprot.org/citations/19619438http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/19619438
http://purl.uniprot.org/citations/19619438http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/19619438
http://purl.uniprot.org/uniprot/#_B4DTD7-mappedCitation-19619438http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19619438
http://purl.uniprot.org/uniprot/#_B4DPS0-mappedCitation-19619438http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19619438
http://purl.uniprot.org/uniprot/#_Q53XS4-mappedCitation-19619438http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19619438
http://purl.uniprot.org/uniprot/#_Q53EL0-mappedCitation-19619438http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19619438
http://purl.uniprot.org/uniprot/#_P29350-mappedCitation-19619438http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19619438
http://purl.uniprot.org/uniprot/Q53XS4http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/19619438
http://purl.uniprot.org/uniprot/B4DTD7http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/19619438
http://purl.uniprot.org/uniprot/P29350http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/19619438
http://purl.uniprot.org/uniprot/B4DPS0http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/19619438
http://purl.uniprot.org/uniprot/Q53EL0http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/19619438
▶