| http://purl.uniprot.org/citations/19623654 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19623654 | http://www.w3.org/2000/01/rdf-schema#comment | "Quantitative detection of minimal residual disease has prognostic value for some leukemias. Acute promyelocytic leukemia (APL) is characterized by the specific PML-RARalpha fusion gene from t(15;17). Added to three PML-RARalpha isoforms, alternative spliced forms of PML exons give rise to multiple isoforms even within a single patient. To date, multiple primer pairs for the detection of the various PML-RARalpha transcripts have been designed, potentially generating some nonspecific amplification products. Here, we established a real-time quantitative PCR (RQ-PCR) strategy with a single primer pair using LightCycler (sp-RQ-PCR), which could simultaneously detect three isoforms with equal specificity and sensitivity as well as alternative spliced forms. Results obtained with sp-RQ-PCR for 39 samples from 15 APL patients and 31 non-APL samples were compared with those with TaqMan assay with three primer pairs. In two of the APL samples, PML-RARalpha was detected in the TM, but not in the sp-RQ-PCR or nested PCR. Furthermore, the sp-RQ-PCR showed no positive results for the 31 non-APL samples, whereas the TM identified 13% (4/31) as positive. Electrophoresis detected some artifacts in the TM, which do not correspond to PML-RARalpha. We conclude that our sp-RQ-PCR is specific enough to identify various forms of PML-RARalpha and yields no false-positive results."xsd:string |
| http://purl.uniprot.org/citations/19623654 | http://purl.org/dc/terms/identifier | "doi:10.1002/jcla.20306"xsd:string |
| http://purl.uniprot.org/citations/19623654 | http://purl.uniprot.org/core/author | "Kawano S."xsd:string |
| http://purl.uniprot.org/citations/19623654 | http://purl.uniprot.org/core/author | "Yoneda K."xsd:string |
| http://purl.uniprot.org/citations/19623654 | http://purl.uniprot.org/core/author | "Kumagai S."xsd:string |
| http://purl.uniprot.org/citations/19623654 | http://purl.uniprot.org/core/author | "Saigo K."xsd:string |
| http://purl.uniprot.org/citations/19623654 | http://purl.uniprot.org/core/author | "Joo K."xsd:string |
| http://purl.uniprot.org/citations/19623654 | http://purl.uniprot.org/core/author | "Nakamachi Y."xsd:string |
| http://purl.uniprot.org/citations/19623654 | http://purl.uniprot.org/core/author | "Tatsumi E."xsd:string |
| http://purl.uniprot.org/citations/19623654 | http://purl.uniprot.org/core/author | "Takenokuchi M."xsd:string |
| http://purl.uniprot.org/citations/19623654 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19623654 | http://purl.uniprot.org/core/name | "J Clin Lab Anal"xsd:string |
| http://purl.uniprot.org/citations/19623654 | http://purl.uniprot.org/core/pages | "223-230"xsd:string |
| http://purl.uniprot.org/citations/19623654 | http://purl.uniprot.org/core/title | "Quantitative detection of PML-RARalpha fusion transcript by real-time PCR with a single primer pair."xsd:string |
| http://purl.uniprot.org/citations/19623654 | http://purl.uniprot.org/core/volume | "23"xsd:string |
| http://purl.uniprot.org/citations/19623654 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19623654 |
| http://purl.uniprot.org/citations/19623654 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19623654 |
| http://purl.uniprot.org/uniprot/#_Q96QH2-mappedCitation-19623654 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19623654 |
| http://purl.uniprot.org/uniprot/Q96QH2 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19623654 |