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http://purl.uniprot.org/citations/19631611http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/19631611http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/19631611http://www.w3.org/2000/01/rdf-schema#comment"The metabolic control of the interaction between ArabidopsisN-acetyl-l-glutamate kinase (NAGK) and the PII protein has been studied. Both gel exclusion and affinity chromatography analyses of recombinant, affinity-purified PII (trimeric complex) and NAGK (hexameric complex) showed that NAGK strongly interacted with PII only in the presence of Mg-ATP, and that this process was reversed by 2-oxoglutarate (2-OG). Furthermore, metabolites such as arginine, glutamate, citrate, and oxalacetate also exerted a negative effect on the PII-NAGK complex formation in the presence of Mg-ATP. Using chloroplast protein extracts and PII affinity chromatography, NAGK interacted with PII only in the presence of ATP-Mg(2+), and this process was antagonized by 2-OG. These results reveal a complex metabolic control of the PII interaction with NAGK in the chloroplast stroma of higher plants."xsd:string
http://purl.uniprot.org/citations/19631611http://purl.org/dc/terms/identifier"doi:10.1016/j.bbrc.2009.07.088"xsd:string
http://purl.uniprot.org/citations/19631611http://purl.org/dc/terms/identifier"doi:10.1016/j.bbrc.2009.07.088"xsd:string
http://purl.uniprot.org/citations/19631611http://purl.uniprot.org/core/author"Hodges M."xsd:string
http://purl.uniprot.org/citations/19631611http://purl.uniprot.org/core/author"Hodges M."xsd:string
http://purl.uniprot.org/citations/19631611http://purl.uniprot.org/core/author"Vidal J."xsd:string
http://purl.uniprot.org/citations/19631611http://purl.uniprot.org/core/author"Vidal J."xsd:string
http://purl.uniprot.org/citations/19631611http://purl.uniprot.org/core/author"Ferrario-Mery S."xsd:string
http://purl.uniprot.org/citations/19631611http://purl.uniprot.org/core/author"Ferrario-Mery S."xsd:string
http://purl.uniprot.org/citations/19631611http://purl.uniprot.org/core/author"Feria Bourrellier A.B."xsd:string
http://purl.uniprot.org/citations/19631611http://purl.uniprot.org/core/author"Feria Bourrellier A.B."xsd:string
http://purl.uniprot.org/citations/19631611http://purl.uniprot.org/core/date"2009"xsd:gYear
http://purl.uniprot.org/citations/19631611http://purl.uniprot.org/core/date"2009"xsd:gYear
http://purl.uniprot.org/citations/19631611http://purl.uniprot.org/core/name"Biochem. Biophys. Res. Commun."xsd:string
http://purl.uniprot.org/citations/19631611http://purl.uniprot.org/core/name"Biochem. Biophys. Res. Commun."xsd:string
http://purl.uniprot.org/citations/19631611http://purl.uniprot.org/core/pages"700-704"xsd:string
http://purl.uniprot.org/citations/19631611http://purl.uniprot.org/core/pages"700-704"xsd:string
http://purl.uniprot.org/citations/19631611http://purl.uniprot.org/core/title"Metabolite regulation of the interaction between Arabidopsis thaliana PII and N-acetyl-l-glutamate kinase."xsd:string
http://purl.uniprot.org/citations/19631611http://purl.uniprot.org/core/title"Metabolite regulation of the interaction between Arabidopsis thaliana PII and N-acetyl-l-glutamate kinase."xsd:string
http://purl.uniprot.org/citations/19631611http://purl.uniprot.org/core/volume"387"xsd:string
http://purl.uniprot.org/citations/19631611http://purl.uniprot.org/core/volume"387"xsd:string
http://purl.uniprot.org/citations/19631611http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/19631611
http://purl.uniprot.org/citations/19631611http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/19631611