| http://purl.uniprot.org/citations/19635745 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19635745 | http://www.w3.org/2000/01/rdf-schema#comment | "Xyloglucan endotransglucosylase/hydrolases (XTHs) are enzymes involved in the modification of load-bearing cell wall components. They cleave xyloglucan chains and, often, re-form bonds to the non-reducing ends of available xyloglucan molecules in plant primary cell walls. The enzymic properties and effects on root growth of two Arabidopsis thaliana XTHs belonging to subgroup I/II, that are predominantly expressed in root hairs and in non-elongating zones of the root, were analysed here. AtXTH14 and AtXTH26 were recombinantly produced in Pichia and subsequently purified. Both proteins were found to exhibit xyloglucan endotransglucosylase (XET; EC 2.4.1.207) but not xyloglucan endohydrolase (XEH; EC 3.2.1.151) activity. Their endotransglucosylase activity was at least 70x greater on xyloglucan rather than on mixed-linkage beta-glucan. Differences were found in pH- and temperature-dependence as well as in acceptor-substrate preferences. Furthermore, the specific activity of XET was approximately equal for the two enzymes. Removal of N-linked sugar residues by Endo H treatment reduced XET activity to 60%. Constant-load extensiometry experiments revealed that the enzymes reduce the extension in a model system of heat-inactivated isolated cell walls. When given to growing roots, either of these XTH proteins reduced cell elongation in a concentration-dependent manner and caused abnormal root hair morphology. This is the first time that recombinant and purified XTHs added to growing roots have exhibited a clear effect on cell elongation. It is proposed that these specific XTH isoenzymes play a role in strengthening the side-walls of root-hairs and cell walls in the root differentiation zone after the completion of cell expansion."xsd:string |
| http://purl.uniprot.org/citations/19635745 | http://purl.org/dc/terms/identifier | "doi:10.1093/jxb/erp229"xsd:string |
| http://purl.uniprot.org/citations/19635745 | http://purl.uniprot.org/core/author | "Verbelen J.P."xsd:string |
| http://purl.uniprot.org/citations/19635745 | http://purl.uniprot.org/core/author | "Vissenberg K."xsd:string |
| http://purl.uniprot.org/citations/19635745 | http://purl.uniprot.org/core/author | "Fry S.C."xsd:string |
| http://purl.uniprot.org/citations/19635745 | http://purl.uniprot.org/core/author | "Maris A."xsd:string |
| http://purl.uniprot.org/citations/19635745 | http://purl.uniprot.org/core/author | "Suslov D."xsd:string |
| http://purl.uniprot.org/citations/19635745 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19635745 | http://purl.uniprot.org/core/name | "J Exp Bot"xsd:string |
| http://purl.uniprot.org/citations/19635745 | http://purl.uniprot.org/core/pages | "3959-3972"xsd:string |
| http://purl.uniprot.org/citations/19635745 | http://purl.uniprot.org/core/title | "Enzymic characterization of two recombinant xyloglucan endotransglucosylase/hydrolase (XTH) proteins of Arabidopsis and their effect on root growth and cell wall extension."xsd:string |
| http://purl.uniprot.org/citations/19635745 | http://purl.uniprot.org/core/volume | "60"xsd:string |
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