| http://purl.uniprot.org/citations/19670267 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19670267 | http://www.w3.org/2000/01/rdf-schema#comment | "Notch signaling plays a key role in osteoblast differentiation. A major transcriptional downstream regulator of this pathway is the helix-loop-helix (HLH) transcription factor Hairy/Enhancer of Split 1 (Hes-1). Here we investigated the function of Hes-1 in osteoblastic cells. Endogenous Hes-1 gene expression decreases during progression of bone cell phenotype development in MC3T3-E1 osteoblasts suggesting that it is a negative regulator of osteoblast differentiation. Forced expression of Hes-1 inhibits osteocalcin (OC) mRNA levels, and luciferase assays indicate that Hes-1 directly represses OC promoter activity. In vitro and in vivo protein/DNA interaction assays reveal that recombinant Hes-1 binds specifically to an E-box in the proximal promoter of the OC gene. Deletion of the Hes-1 WRPW domain (MHes-1) that recruits the co-repressor Groucho abrogates repression of OC promoter activity by Hes-1, but also blocks Hes-1 binding to the promoter. The latter result suggests that exogenous Hes-1 may be recruited to the OC promoter by both protein/DNA and protein/protein interactions. We conclude that the Notch-responsive Hes-1 protein is capable of repressing OC gene transcription in osteoblastic cells through an E-box in the proximal promoter. Hes-1 may contribute to osteoblast growth and differentiation by controlling basal bone-specific transcription directly through interactions with transcriptional regulators that are known to bind to the OC gene promoter."xsd:string |
| http://purl.uniprot.org/citations/19670267 | http://purl.org/dc/terms/identifier | "doi:10.1002/jcb.22299"xsd:string |
| http://purl.uniprot.org/citations/19670267 | http://purl.uniprot.org/core/author | "Zhang Y."xsd:string |
| http://purl.uniprot.org/citations/19670267 | http://purl.uniprot.org/core/author | "Stein G.S."xsd:string |
| http://purl.uniprot.org/citations/19670267 | http://purl.uniprot.org/core/author | "Stein J.L."xsd:string |
| http://purl.uniprot.org/citations/19670267 | http://purl.uniprot.org/core/author | "van Wijnen A.J."xsd:string |
| http://purl.uniprot.org/citations/19670267 | http://purl.uniprot.org/core/author | "Lian J.B."xsd:string |
| http://purl.uniprot.org/citations/19670267 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19670267 | http://purl.uniprot.org/core/name | "J Cell Biochem"xsd:string |
| http://purl.uniprot.org/citations/19670267 | http://purl.uniprot.org/core/pages | "651-659"xsd:string |
| http://purl.uniprot.org/citations/19670267 | http://purl.uniprot.org/core/title | "The Notch-responsive transcription factor Hes-1 attenuates osteocalcin promoter activity in osteoblastic cells."xsd:string |
| http://purl.uniprot.org/citations/19670267 | http://purl.uniprot.org/core/volume | "108"xsd:string |
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