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| http://purl.uniprot.org/citations/1967079 | http://www.w3.org/2000/01/rdf-schema#comment | "An affinity chromatographic matrix that purifies cellular retinoic acid-binding protein to near homogeneity from rat testes cytosol has been developed. The three-step procedure includes an acid precipitation, a batch treatment with CM Bio-Gel, and affinity chromatography on 4-(2-hydroxyethoxy)retinoic acid coupled to epoxy-activated Sepharose 6B. The binding protein was purified approximately 8500-fold based on total soluble testicular protein and with a recovery in excess of 80%. In addition, further enhancement of the purity of the protein can be attained by size-exclusion HPLC to increase purification to 21,000-fold. The recovered protein has an apparent M(r) 14,300 as determined by size-exclusion HPLC and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The protein is isolated in the apo-form and retains its ability to bind retinoic acid as evidenced by the binding of [3H]retinoic acid. An apparent retinoic acid-binding protein of M(r) 18,000 has also been isolated from rat testes nuclei by the affinity chromatography step. The affinity phase has been used for 6 months without any detectable loss in its ability to purify cellular retinoic acid-binding protein."xsd:string |
| http://purl.uniprot.org/citations/1967079 | http://purl.org/dc/terms/identifier | "doi:10.1016/1046-5928(90)90047-3"xsd:string |
| http://purl.uniprot.org/citations/1967079 | http://purl.uniprot.org/core/author | "Chapman J.M."xsd:string |
| http://purl.uniprot.org/citations/1967079 | http://purl.uniprot.org/core/author | "Curley R.W. Jr."xsd:string |
| http://purl.uniprot.org/citations/1967079 | http://purl.uniprot.org/core/date | "1990"xsd:gYear |
| http://purl.uniprot.org/citations/1967079 | http://purl.uniprot.org/core/name | "Protein Expr Purif"xsd:string |
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| http://purl.uniprot.org/citations/1967079 | http://purl.uniprot.org/core/title | "Affinity purification of retinoic acid-binding proteins using immobilized 4-(2-hydroxyethoxy)retinoic acid."xsd:string |
| http://purl.uniprot.org/citations/1967079 | http://purl.uniprot.org/core/volume | "1"xsd:string |
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