| http://purl.uniprot.org/citations/19675099 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19675099 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19675099 | http://www.w3.org/2000/01/rdf-schema#comment | "Upon activation, NF-kappaB translocates into the nucleus and initiates biological events. This NF-kappaB signalling is mainly regulated by the protein kinase IKKbeta. Early in this signalling pathway, IKKbeta is phosphorylated for activation by several factors, such as pro-inflammatory cytokines and the Tax oncoprotein of HTLV-1. In cells infected by HTLV-1, IKKbeta is persistently phosphorylated and conjugated with monoubiquitin due to Tax expression. Although this Tax-induced monoubiquitination appears to be an important regulation system for IKKbeta, how the monoubiquitination occurs is unknown and its role in NF-kappaB signalling is still unclear. Here, we show that an E3-ubiquitin ligase Ro52 interacts weakly with wild-type IKKbeta but strongly with a phosphomimetic mutant IKKbeta to conjugate monoubiquitin in cooperation with an E2-ubiquitin-conjugating enzyme UbcH5B. These results suggest that the Tax-induced phosphorylation of IKKbeta causes an interaction with Ro52 for the subsequent monoubiquitination. NF-kappaB reporter assays have shown that the IKKbeta activity is suppressed by wild-type Ro52, but not by its inactive mutant. In addition, monoubiquitin fusion of IKKbeta reduced its activity for NF-kappaB signalling. We also found that Ro52 dramatically reduces the level of Tax. These results suggest that Ro52 down-regulates Tax-induced NF-kappaB signalling by monoubiquitinating IKKbeta and by reducing the level of Tax."xsd:string |
| http://purl.uniprot.org/citations/19675099 | http://purl.org/dc/terms/identifier | "doi:10.1093/jb/mvp127"xsd:string |
| http://purl.uniprot.org/citations/19675099 | http://purl.org/dc/terms/identifier | "doi:10.1093/jb/mvp127"xsd:string |
| http://purl.uniprot.org/citations/19675099 | http://purl.uniprot.org/core/author | "Wada K."xsd:string |
| http://purl.uniprot.org/citations/19675099 | http://purl.uniprot.org/core/author | "Wada K."xsd:string |
| http://purl.uniprot.org/citations/19675099 | http://purl.uniprot.org/core/author | "Tanaka M."xsd:string |
| http://purl.uniprot.org/citations/19675099 | http://purl.uniprot.org/core/author | "Tanaka M."xsd:string |
| http://purl.uniprot.org/citations/19675099 | http://purl.uniprot.org/core/author | "Kamitani T."xsd:string |
| http://purl.uniprot.org/citations/19675099 | http://purl.uniprot.org/core/author | "Kamitani T."xsd:string |
| http://purl.uniprot.org/citations/19675099 | http://purl.uniprot.org/core/author | "Niida M."xsd:string |
| http://purl.uniprot.org/citations/19675099 | http://purl.uniprot.org/core/author | "Niida M."xsd:string |
| http://purl.uniprot.org/citations/19675099 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19675099 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19675099 | http://purl.uniprot.org/core/name | "J. Biochem."xsd:string |
| http://purl.uniprot.org/citations/19675099 | http://purl.uniprot.org/core/name | "J. Biochem."xsd:string |
| http://purl.uniprot.org/citations/19675099 | http://purl.uniprot.org/core/pages | "821-832"xsd:string |
| http://purl.uniprot.org/citations/19675099 | http://purl.uniprot.org/core/pages | "821-832"xsd:string |
| http://purl.uniprot.org/citations/19675099 | http://purl.uniprot.org/core/title | "Ro52-mediated monoubiquitination of IKK{beta} down-regulates NF-{kappa}B signalling."xsd:string |
| http://purl.uniprot.org/citations/19675099 | http://purl.uniprot.org/core/title | "Ro52-mediated monoubiquitination of IKK{beta} down-regulates NF-{kappa}B signalling."xsd:string |
| http://purl.uniprot.org/citations/19675099 | http://purl.uniprot.org/core/volume | "146"xsd:string |
| http://purl.uniprot.org/citations/19675099 | http://purl.uniprot.org/core/volume | "146"xsd:string |
| http://purl.uniprot.org/citations/19675099 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19675099 |
| http://purl.uniprot.org/citations/19675099 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19675099 |