| http://purl.uniprot.org/citations/19675172 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19675172 | http://www.w3.org/2000/01/rdf-schema#comment | "During the recall response by CD27(+) IgG class-switched human memory B cells, total IgG secreted is a function of the following: 1) the number of IgG-secreting cells (IgG-SC), and 2) the secretion rate of each cell. In this study, we report the quantitative ELISPOT method for simultaneous estimation of single-cell IgG secretion rates and secreting cell frequencies in human B cell populations. We found that CD27(+) IgM(-) memory B cells activated with CpG and cytokines had considerable heterogeneity in the IgG secretion rates, with two major secretion rate subpopulations. BCR cross-linking reduced the frequency of cells with high per-cell IgG secretion rates, with a parallel decrease in CD27(high) B cell blasts. Increased cell death may account for the BCR-stimulated reduction in high-rate IgG-SC CD27(high) B cell blasts. In contrast, the addition of IL-21 to CD40L plus IL-4-activated human memory B cells induced a high-rate IgG-SC population in B cells with otherwise low per-cell IgG secretion rates. The profiles of human B cell IgG secretion rates followed the same biphasic distribution and range irrespective of division class. This, along with the presence of non-IgG-producing, dividing B cells in CpG plus cytokine-activated B memory B cell populations, is suggestive of an on/off switch regulating IgG secretion. Finally, these data support a mixture model of IgG secretion in which IgG secreted over time is modulated by the frequency of IgG-SC and the distribution of their IgG secretion rates."xsd:string |
| http://purl.uniprot.org/citations/19675172 | http://purl.org/dc/terms/identifier | "doi:10.4049/jimmunol.0804233"xsd:string |
| http://purl.uniprot.org/citations/19675172 | http://purl.uniprot.org/core/author | "Brown M.A."xsd:string |
| http://purl.uniprot.org/citations/19675172 | http://purl.uniprot.org/core/author | "Murphy A.J."xsd:string |
| http://purl.uniprot.org/citations/19675172 | http://purl.uniprot.org/core/author | "Pellegrin T."xsd:string |
| http://purl.uniprot.org/citations/19675172 | http://purl.uniprot.org/core/author | "Hyrien O."xsd:string |
| http://purl.uniprot.org/citations/19675172 | http://purl.uniprot.org/core/author | "Mosmann T."xsd:string |
| http://purl.uniprot.org/citations/19675172 | http://purl.uniprot.org/core/author | "Henn A.D."xsd:string |
| http://purl.uniprot.org/citations/19675172 | http://purl.uniprot.org/core/author | "Zand M.S."xsd:string |
| http://purl.uniprot.org/citations/19675172 | http://purl.uniprot.org/core/author | "Coca M.N."xsd:string |
| http://purl.uniprot.org/citations/19675172 | http://purl.uniprot.org/core/author | "Rebhahn J."xsd:string |
| http://purl.uniprot.org/citations/19675172 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19675172 | http://purl.uniprot.org/core/name | "J Immunol"xsd:string |
| http://purl.uniprot.org/citations/19675172 | http://purl.uniprot.org/core/pages | "3177-3187"xsd:string |
| http://purl.uniprot.org/citations/19675172 | http://purl.uniprot.org/core/title | "Modulation of single-cell IgG secretion frequency and rates in human memory B cells by CpG DNA, CD40L, IL-21, and cell division."xsd:string |
| http://purl.uniprot.org/citations/19675172 | http://purl.uniprot.org/core/volume | "183"xsd:string |
| http://purl.uniprot.org/citations/19675172 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19675172 |
| http://purl.uniprot.org/citations/19675172 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19675172 |
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| http://purl.uniprot.org/uniprot/#_Q3L8U2-mappedCitation-19675172 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19675172 |
| http://purl.uniprot.org/uniprot/#_Q16063-mappedCitation-19675172 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19675172 |
| http://purl.uniprot.org/uniprot/#_P29965-mappedCitation-19675172 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19675172 |
| http://purl.uniprot.org/uniprot/#_Q45QX2-mappedCitation-19675172 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19675172 |