| http://purl.uniprot.org/citations/19683723 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19683723 | http://www.w3.org/2000/01/rdf-schema#comment | "Na,K-ATPase is composed of two essential alpha- and beta-subunits, both of which have multiple isoforms. Evidence indicates that the Na,K-ATPase enzymatic activity as well as its alpha(1), alpha(3) and beta(1) isoforms are reduced in the failing human heart. The catalytic alpha-subunit is the receptor for cardiac glycosides such as digitalis, used for the treatment of congestive heart failure. The role of the Na,K-ATPase beta(1)-subunit (Na,K-beta(1)) in cardiac function is not known. We used Cre/loxP technology to inactivate the Na,K-beta(1) gene exclusively in the ventricular cardiomyocytes. Animals with homozygous Na,K-beta(1) gene excision were born at the expected Mendelian ratio, grew into adulthood, and appeared to be healthy until 10 months of age. At 13-14 months, these mice had 13% higher heart/body weight ratios, and reduced contractility as revealed by echocardiography compared to their wild-type (WT) littermates. Pressure overload by transverse aortic constriction (TAC) in younger mice, resulted in compensated hypertrophy in WT mice, but decompensation in the Na,K-beta(1) KO mice. The young KO survivors of TAC exhibited decreased contractile function and mimicked the effects of the Na,K-beta(1) KO in older mice. Further, we show that intact hearts of Na,K-beta(1) KO anesthetized mice as well as isolated cardiomyocytes were insensitive to ouabain-induced positive inotropy. This insensitivity was associated with a reduction in NCX1, one of the proteins involved in regulating cardiac contractility. In conclusion, our results demonstrate that Na,K-beta(1) plays an essential role in regulating cardiac contractility and that its loss is associated with significant pathophysiology of the heart."xsd:string |
| http://purl.uniprot.org/citations/19683723 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.yjmcc.2009.07.018"xsd:string |
| http://purl.uniprot.org/citations/19683723 | http://purl.uniprot.org/core/author | "Fang K."xsd:string |
| http://purl.uniprot.org/citations/19683723 | http://purl.uniprot.org/core/author | "Johnson C.L."xsd:string |
| http://purl.uniprot.org/citations/19683723 | http://purl.uniprot.org/core/author | "Barwe S.P."xsd:string |
| http://purl.uniprot.org/citations/19683723 | http://purl.uniprot.org/core/author | "Rajasekaran A.K."xsd:string |
| http://purl.uniprot.org/citations/19683723 | http://purl.uniprot.org/core/author | "Rajasekaran S.A."xsd:string |
| http://purl.uniprot.org/citations/19683723 | http://purl.uniprot.org/core/author | "Wolle D."xsd:string |
| http://purl.uniprot.org/citations/19683723 | http://purl.uniprot.org/core/author | "Rozengurt N."xsd:string |
| http://purl.uniprot.org/citations/19683723 | http://purl.uniprot.org/core/author | "Goldhaber J.I."xsd:string |
| http://purl.uniprot.org/citations/19683723 | http://purl.uniprot.org/core/author | "Jordan M.C."xsd:string |
| http://purl.uniprot.org/citations/19683723 | http://purl.uniprot.org/core/author | "Roos K.P."xsd:string |
| http://purl.uniprot.org/citations/19683723 | http://purl.uniprot.org/core/author | "Inge L."xsd:string |
| http://purl.uniprot.org/citations/19683723 | http://purl.uniprot.org/core/author | "Neco P."xsd:string |
| http://purl.uniprot.org/citations/19683723 | http://purl.uniprot.org/core/author | "Skay A."xsd:string |
| http://purl.uniprot.org/citations/19683723 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19683723 | http://purl.uniprot.org/core/name | "J Mol Cell Cardiol"xsd:string |
| http://purl.uniprot.org/citations/19683723 | http://purl.uniprot.org/core/pages | "552-560"xsd:string |
| http://purl.uniprot.org/citations/19683723 | http://purl.uniprot.org/core/title | "Dysfunction of ouabain-induced cardiac contractility in mice with heart-specific ablation of Na,K-ATPase beta1-subunit."xsd:string |
| http://purl.uniprot.org/citations/19683723 | http://purl.uniprot.org/core/volume | "47"xsd:string |
| http://purl.uniprot.org/citations/19683723 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19683723 |
| http://purl.uniprot.org/citations/19683723 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19683723 |
| http://purl.uniprot.org/uniprot/P13637#attribution-8F5C9ADF2E11CD3FB8D3E5E70307192B | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/19683723 |
| http://purl.uniprot.org/uniprot/P05023#attribution-8F5C9ADF2E11CD3FB8D3E5E70307192B | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/19683723 |