| http://purl.uniprot.org/citations/19690090 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19690090 | http://www.w3.org/2000/01/rdf-schema#comment | "Galbeta1-4GlcNAc is thought to be a common disaccharide unit preferentially recognized by vertebrate galectins. Eight-amino-acid residues conserved in proteins belonging to the galectin family have been suggested to be responsible for recognition. Meanwhile, we isolated and analyzed endogenous N-glycans of Caenorhabditis elegans that were captured by a C. elegans galectin LEC-6 and demonstrated that the unit of recognition for LEC-6 is a Gal-Fuc disaccharide, though the linkage between these residues was not confirmed. In the present study, we chemically synthesized Galbeta1-4Fuc and Galbeta1-3Fuc labeled with 2-aminopyridine (PA) and demonstrated that LEC-6 interacts with PA-Galbeta1-4Fuc more strongly than PA-Galbeta1-3Fuc by frontal affinity chromatography (FAC). Galbeta1-4Fuc also inhibited hemagglutination caused by LEC-6 more strongly than Galbeta1-3Fuc. FAC analysis using LEC-6 point mutants revealed that some of the conserved amino acid residues which have proven to be important for the recognition of Galbeta1-4GlcNAc are not necessary for the binding to Galbeta1-4Fuc. Another major C. elegans galectin, LEC-1, also showed preferential binding to Galbeta1-4Fuc. These results suggest that Galbeta1-4Fuc is the endogenous unit structure recognized by C. elegans galectins, which implies that C. elegans glycans and galectins may have co-evolved through an alteration in the structures of C. elegans glycans and a subsequent conversion in the sugar-binding mechanism of galectins. Furthermore, since glycans containing the Galbeta1-4Fuc disaccharide unit have been found in organisms belonging to Protostomia, this unit might be a common glyco-epitope recognized by galectins in these organisms."xsd:string |
| http://purl.uniprot.org/citations/19690090 | http://purl.org/dc/terms/identifier | "doi:10.1093/glycob/cwp125"xsd:string |
| http://purl.uniprot.org/citations/19690090 | http://purl.uniprot.org/core/author | "Kobayashi S."xsd:string |
| http://purl.uniprot.org/citations/19690090 | http://purl.uniprot.org/core/author | "Takahashi H."xsd:string |
| http://purl.uniprot.org/citations/19690090 | http://purl.uniprot.org/core/author | "Takahashi M."xsd:string |
| http://purl.uniprot.org/citations/19690090 | http://purl.uniprot.org/core/author | "Sugiura K."xsd:string |
| http://purl.uniprot.org/citations/19690090 | http://purl.uniprot.org/core/author | "Yamada A."xsd:string |
| http://purl.uniprot.org/citations/19690090 | http://purl.uniprot.org/core/author | "Takeuchi T."xsd:string |
| http://purl.uniprot.org/citations/19690090 | http://purl.uniprot.org/core/author | "Kasai K."xsd:string |
| http://purl.uniprot.org/citations/19690090 | http://purl.uniprot.org/core/author | "Nishiyama K."xsd:string |
| http://purl.uniprot.org/citations/19690090 | http://purl.uniprot.org/core/author | "Natsugari H."xsd:string |
| http://purl.uniprot.org/citations/19690090 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19690090 | http://purl.uniprot.org/core/name | "Glycobiology"xsd:string |
| http://purl.uniprot.org/citations/19690090 | http://purl.uniprot.org/core/pages | "1503-1510"xsd:string |
| http://purl.uniprot.org/citations/19690090 | http://purl.uniprot.org/core/title | "Caenorhabditis elegans galectins LEC-6 and LEC-1 recognize a chemically synthesized Galbeta1-4Fuc disaccharide unit which is present in Protostomia glycoconjugates."xsd:string |
| http://purl.uniprot.org/citations/19690090 | http://purl.uniprot.org/core/volume | "19"xsd:string |
| http://purl.uniprot.org/citations/19690090 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19690090 |
| http://purl.uniprot.org/citations/19690090 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19690090 |
| http://purl.uniprot.org/uniprot/#_O45904-mappedCitation-19690090 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19690090 |
| http://purl.uniprot.org/uniprot/#_P36573-mappedCitation-19690090 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19690090 |
| http://purl.uniprot.org/uniprot/#_Q9N384-mappedCitation-19690090 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19690090 |
| http://purl.uniprot.org/uniprot/O45904 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19690090 |
| http://purl.uniprot.org/uniprot/P36573 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19690090 |
| http://purl.uniprot.org/uniprot/Q9N384 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19690090 |