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http://purl.uniprot.org/citations/19692427http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/19692427http://www.w3.org/2000/01/rdf-schema#comment"Numerous cellular processes are regulated by the reversible addition of either phosphate or O-linked beta-N-acetylglucosamine (O-GlcNAc) to nuclear and cytoplasmic proteins. Although sensitive methods exist for the enrichment and identification of protein phosphorylation sites, those for the enrichment of O-GlcNAc-containing peptides are lacking. Reported here is highly efficient methodology for the enrichment and characterization of O-GlcNAc sites from complex samples. In this method, O-GlcNAc-modified peptides are tagged with a novel biotinylation reagent, enriched by affinity chromatography, released from the solid support by photochemical cleavage, and analyzed by electron transfer dissociation mass spectrometry. Using this strategy, eight O-GlcNAc sites were mapped from a tau-enriched sample from rat brain. Sites of GlcNAcylation were characterized on important neuronal proteins such as tau, synucleins, and methyl CpG-binding protein 2."xsd:string
http://purl.uniprot.org/citations/19692427http://purl.org/dc/terms/identifier"doi:10.1074/mcp.m900268-mcp200"xsd:string
http://purl.uniprot.org/citations/19692427http://purl.uniprot.org/core/author"Hunt D.F."xsd:string
http://purl.uniprot.org/citations/19692427http://purl.uniprot.org/core/author"Shabanowitz J."xsd:string
http://purl.uniprot.org/citations/19692427http://purl.uniprot.org/core/author"Wang Z."xsd:string
http://purl.uniprot.org/citations/19692427http://purl.uniprot.org/core/author"Hart G.W."xsd:string
http://purl.uniprot.org/citations/19692427http://purl.uniprot.org/core/author"Udeshi N.D."xsd:string
http://purl.uniprot.org/citations/19692427http://purl.uniprot.org/core/author"O'Malley M."xsd:string
http://purl.uniprot.org/citations/19692427http://purl.uniprot.org/core/date"2010"xsd:gYear
http://purl.uniprot.org/citations/19692427http://purl.uniprot.org/core/name"Mol Cell Proteomics"xsd:string
http://purl.uniprot.org/citations/19692427http://purl.uniprot.org/core/pages"153-160"xsd:string
http://purl.uniprot.org/citations/19692427http://purl.uniprot.org/core/title"Enrichment and site mapping of O-linked N-acetylglucosamine by a combination of chemical/enzymatic tagging, photochemical cleavage, and electron transfer dissociation mass spectrometry."xsd:string
http://purl.uniprot.org/citations/19692427http://purl.uniprot.org/core/volume"9"xsd:string
http://purl.uniprot.org/citations/19692427http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/19692427
http://purl.uniprot.org/citations/19692427http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/19692427
http://purl.uniprot.org/uniprot/#_A0A8J8XUY4-mappedCitation-19692427http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19692427
http://purl.uniprot.org/uniprot/#_A0A8K1TMD4-mappedCitation-19692427http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19692427
http://purl.uniprot.org/uniprot/#_A0A8K1TN53-mappedCitation-19692427http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19692427
http://purl.uniprot.org/uniprot/#_A0A8K1WG57-mappedCitation-19692427http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19692427
http://purl.uniprot.org/uniprot/#_A0A8K1WHA3-mappedCitation-19692427http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19692427
http://purl.uniprot.org/uniprot/#_A0A2Z5HTN7-mappedCitation-19692427http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19692427
http://purl.uniprot.org/uniprot/#_A6HJU3-mappedCitation-19692427http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19692427
http://purl.uniprot.org/uniprot/#_A6HJU8-mappedCitation-19692427http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19692427
http://purl.uniprot.org/uniprot/#_A6HJU9-mappedCitation-19692427http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19692427