| http://purl.uniprot.org/citations/19706303 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19706303 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19706303 | http://www.w3.org/2000/01/rdf-schema#comment | "In view of the findings that several Kunitz-type protein inhibitors suppress tumor invasion and metastasis, the aim of the present study is to explore whether Bungarus multicinctus protease inhibitor-like protein-2 (PILP-2) and PILP-3 exhibit anti-tumor activity. Although approximately 28% of amino acid substitutions occurred between PILP-2 and PILP-3, molecular modeling suggested that PILP-2 and PILP-3 shared similar folded structures. Unlike PILP-2, PILP-3 showed a notable activity in abolishing migration and invasion of human neuroblastoma SK-N-SH cells. The ability of PILP-3 to inhibit matrix metalloprotease-2 (MMP-2) activity was higher than that of PILP-2. Pull-down assay revealed protein-protein interaction between PILP-3 and MMP-2. In contrast to mutation on N-terminal region, replacement of amino acids at C-terminus attenuated notably the ability of PILP-3 to inhibit cell invasion, cell migration and MMP-2 activity as well as the binding capability of PILP-3 with MMP-2. Molecular docking showed that N-terminal region of PILP-2 and PILP-3 fitted into the cleft around the active site of MMP-2 catalytic domain. In contrast to that of PILP-2, C-terminal region of PILP-3 was suggested to be in close contact with catalytic domain of MMP-2. Collectively, our data indicate that PILP-3 is a MMP-2 inhibitor and shows an activity in inhibiting migration and invasion of neuroblastoma, and suggest that intact C-terminus is crucial to the activities of PILP-3."xsd:string |
| http://purl.uniprot.org/citations/19706303 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.toxicon.2009.08.012"xsd:string |
| http://purl.uniprot.org/citations/19706303 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.toxicon.2009.08.012"xsd:string |
| http://purl.uniprot.org/citations/19706303 | http://purl.uniprot.org/core/author | "Chang L.S."xsd:string |
| http://purl.uniprot.org/citations/19706303 | http://purl.uniprot.org/core/author | "Chang L.S."xsd:string |
| http://purl.uniprot.org/citations/19706303 | http://purl.uniprot.org/core/author | "Chen K.C."xsd:string |
| http://purl.uniprot.org/citations/19706303 | http://purl.uniprot.org/core/author | "Chen K.C."xsd:string |
| http://purl.uniprot.org/citations/19706303 | http://purl.uniprot.org/core/author | "Liu W.H."xsd:string |
| http://purl.uniprot.org/citations/19706303 | http://purl.uniprot.org/core/author | "Liu W.H."xsd:string |
| http://purl.uniprot.org/citations/19706303 | http://purl.uniprot.org/core/author | "Chou W.M."xsd:string |
| http://purl.uniprot.org/citations/19706303 | http://purl.uniprot.org/core/author | "Chou W.M."xsd:string |
| http://purl.uniprot.org/citations/19706303 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/19706303 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/19706303 | http://purl.uniprot.org/core/name | "Toxicon"xsd:string |
| http://purl.uniprot.org/citations/19706303 | http://purl.uniprot.org/core/name | "Toxicon"xsd:string |
| http://purl.uniprot.org/citations/19706303 | http://purl.uniprot.org/core/pages | "353-360"xsd:string |
| http://purl.uniprot.org/citations/19706303 | http://purl.uniprot.org/core/pages | "353-360"xsd:string |
| http://purl.uniprot.org/citations/19706303 | http://purl.uniprot.org/core/title | "Structure-function studies on inhibitory activity of Bungarus multicinctus protease inhibitor-like protein on matrix metalloprotease-2, and invasion and migration of human neuroblastoma SK-N-SH cells."xsd:string |
| http://purl.uniprot.org/citations/19706303 | http://purl.uniprot.org/core/title | "Structure-function studies on inhibitory activity of Bungarus multicinctus protease inhibitor-like protein on matrix metalloprotease-2, and invasion and migration of human neuroblastoma SK-N-SH cells."xsd:string |
| http://purl.uniprot.org/citations/19706303 | http://purl.uniprot.org/core/volume | "55"xsd:string |
| http://purl.uniprot.org/citations/19706303 | http://purl.uniprot.org/core/volume | "55"xsd:string |
| http://purl.uniprot.org/citations/19706303 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19706303 |
| http://purl.uniprot.org/citations/19706303 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19706303 |