| http://purl.uniprot.org/citations/19706593 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19706593 | http://www.w3.org/2000/01/rdf-schema#comment | "alpha-Hemoglobin (alphaHb) stabilizing protein (AHSP) is expressed in erythropoietic tissues as an accessory factor in hemoglobin synthesis. AHSP forms a specific complex with alphaHb and suppresses the heme-catalyzed evolution of reactive oxygen species by converting alphaHb to a conformation in which the heme is coordinated at both axial positions by histidine side chains (bis-histidyl coordination). Currently, the detailed mechanism by which AHSP induces structural changes in alphaHb has not been determined. Here, we present x-ray crystallography, NMR spectroscopy, and mutagenesis data that identify, for the first time, the importance of an evolutionarily conserved proline, Pro(30), in loop 1 of AHSP. Mutation of Pro(30) to a variety of residue types results in reduced ability to convert alphaHb. In complex with alphaHb, AHSP Pro(30) adopts a cis-peptidyl conformation and makes contact with the N terminus of helix G in alphaHb. Mutations that stabilize the cis-peptidyl conformation of free AHSP, also enhance the alphaHb conversion activity. These findings suggest that AHSP loop 1 can transmit structural changes to the heme pocket of alphaHb, and, more generally, highlight the importance of cis-peptidyl prolyl residues in defining the conformation of regulatory protein loops."xsd:string |
| http://purl.uniprot.org/citations/19706593 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m109.027045"xsd:string |
| http://purl.uniprot.org/citations/19706593 | http://purl.uniprot.org/core/author | "Feng L."xsd:string |
| http://purl.uniprot.org/citations/19706593 | http://purl.uniprot.org/core/author | "Jeffrey P.D."xsd:string |
| http://purl.uniprot.org/citations/19706593 | http://purl.uniprot.org/core/author | "Shi Y."xsd:string |
| http://purl.uniprot.org/citations/19706593 | http://purl.uniprot.org/core/author | "Zhou S."xsd:string |
| http://purl.uniprot.org/citations/19706593 | http://purl.uniprot.org/core/author | "Bendak K."xsd:string |
| http://purl.uniprot.org/citations/19706593 | http://purl.uniprot.org/core/author | "Weiss M.J."xsd:string |
| http://purl.uniprot.org/citations/19706593 | http://purl.uniprot.org/core/author | "Mackay J.P."xsd:string |
| http://purl.uniprot.org/citations/19706593 | http://purl.uniprot.org/core/author | "Gell D.A."xsd:string |
| http://purl.uniprot.org/citations/19706593 | http://purl.uniprot.org/core/author | "Gow A."xsd:string |
| http://purl.uniprot.org/citations/19706593 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19706593 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/19706593 | http://purl.uniprot.org/core/pages | "29462-29469"xsd:string |
| http://purl.uniprot.org/citations/19706593 | http://purl.uniprot.org/core/title | "A cis-proline in alpha-hemoglobin stabilizing protein directs the structural reorganization of alpha-hemoglobin."xsd:string |
| http://purl.uniprot.org/citations/19706593 | http://purl.uniprot.org/core/volume | "284"xsd:string |
| http://purl.uniprot.org/citations/19706593 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19706593 |
| http://purl.uniprot.org/citations/19706593 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19706593 |
| http://purl.uniprot.org/uniprot/#_P69905-mappedCitation-19706593 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19706593 |
| http://purl.uniprot.org/uniprot/#_Q549J4-mappedCitation-19706593 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19706593 |
| http://purl.uniprot.org/uniprot/#_Q9NZD4-mappedCitation-19706593 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19706593 |
| http://purl.uniprot.org/uniprot/P69905 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19706593 |
| http://purl.uniprot.org/uniprot/Q549J4 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19706593 |
| http://purl.uniprot.org/uniprot/Q9NZD4 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19706593 |