| http://purl.uniprot.org/citations/19710202 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19710202 | http://www.w3.org/2000/01/rdf-schema#comment | "The chicken ovary is organized into a hierarchy of yellow yolky follicles that ovulate on successive days. Active or passive immunization of laying hens against testosterone blocks ovulation without affecting follicle development. Testosterone may play a role in pre-ovulatory follicle maturation by stimulating granulosa progesterone production. We assessed whether this stimulus is dose-related and depends on the maturity of the donor follicle, and if it does so by stimulating granulosa cell STAR, P450 cholesterol side-chain cleavage (P450scc), and LH receptor (LHCGR) mRNAs expression. Progesterone production by granulosa cells from F1, F3, and F4 follicles, cultured for 3 h without testosterone was greater in cells collected 11-14 h than 1-4 h after ovulation. These differences in progesterone production were less pronounced after granulosa cells had been cultured for 24 h. Culture of granulosa cells for 3 or 24 h with testosterone (1-100 ng/ml) stimulated progesterone production in cells collected from F4, F3, or F1 follicles 1-4, or 11-14 h after ovulation. Testosterone (0-4000 ng/ml) alone or in combination with LH (0-100 ng/ml) increased progesterone production by F1 granulosa cells, collected 1-4 and 11-14 h after ovulation and cultured for 3 h. Finally, testosterone (10 or 100 ng/ml) increased STAR, P450scc, and LHCGR mRNAs, when added to 3 h cultures of F1 granulosa cells. In conclusion, testosterone stimulates granulosa cell progesterone production in hen pre-ovulatory hierarchical follicles irrespective of maturational state, acting alone or additively with LH. We propose that testosterone promotes granulosa cell maturation to facilitate the pre-ovulatory release of LH."xsd:string |
| http://purl.uniprot.org/citations/19710202 | http://purl.org/dc/terms/identifier | "doi:10.1530/rep-09-0071"xsd:string |
| http://purl.uniprot.org/citations/19710202 | http://purl.uniprot.org/core/author | "Rodriguez A."xsd:string |
| http://purl.uniprot.org/citations/19710202 | http://purl.uniprot.org/core/author | "Sharp P.J."xsd:string |
| http://purl.uniprot.org/citations/19710202 | http://purl.uniprot.org/core/author | "Rojas S."xsd:string |
| http://purl.uniprot.org/citations/19710202 | http://purl.uniprot.org/core/author | "Gutierrez C.G."xsd:string |
| http://purl.uniprot.org/citations/19710202 | http://purl.uniprot.org/core/author | "Rangel P.L."xsd:string |
| http://purl.uniprot.org/citations/19710202 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19710202 | http://purl.uniprot.org/core/name | "Reproduction"xsd:string |
| http://purl.uniprot.org/citations/19710202 | http://purl.uniprot.org/core/pages | "961-969"xsd:string |
| http://purl.uniprot.org/citations/19710202 | http://purl.uniprot.org/core/title | "Testosterone stimulates progesterone production and STAR, P450 cholesterol side-chain cleavage and LH receptor mRNAs expression in hen (Gallus domesticus) granulosa cells."xsd:string |
| http://purl.uniprot.org/citations/19710202 | http://purl.uniprot.org/core/volume | "138"xsd:string |
| http://purl.uniprot.org/citations/19710202 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19710202 |
| http://purl.uniprot.org/citations/19710202 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19710202 |
| http://purl.uniprot.org/uniprot/#_O13254-mappedCitation-19710202 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19710202 |
| http://purl.uniprot.org/uniprot/#_Q90674-mappedCitation-19710202 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19710202 |
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| http://purl.uniprot.org/uniprot/Q90674 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19710202 |
| http://purl.uniprot.org/uniprot/O13254 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19710202 |
| http://purl.uniprot.org/uniprot/Q9DER3 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19710202 |