| http://purl.uniprot.org/citations/19712110 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19712110 | http://www.w3.org/2000/01/rdf-schema#comment | "The bacterial tetracycline transcription regulation system mediated by the tetracycline repressor (TetR) is widely used to study gene expression in prokaryotes and eukaryotes. To study multiple genes in parallel, a triple mutant TetR(K(64)L(135)I(138)) has been engineered that is selectively induced by the synthetic tetracycline derivative 4-de-dimethylamino-anhydrotetracycline (4-ddma-atc) and no longer by tetracycline, the inducer of wild-type TetR. In the present study, we report the crystal structure of TetR(K(64)L(135)I(138)) in the absence and in complex with 4-ddma-atc at resolutions of 2.1 A. Analysis of the structures in light of the available binding data and previously reported TetR complexes allows for a dissection of the origins of selectivity and specificity. In all crystal structures solved to date, the ligand-binding position, as well as the positioning of the residues lining the binding site, is extremely well conserved, irrespective of the chemical nature of the ligand. Selective recognition of 4-ddma-atc is achieved through fine-tuned hydrogen-bonding constraints introduced by the His64-->Lys substitution, as well as a combination of hydrophobic effect and the removal of unfavorable electrostatic interactions through the introduction of Leu135 and Ile138."xsd:string |
| http://purl.uniprot.org/citations/19712110 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1742-4658.2009.07254.x"xsd:string |
| http://purl.uniprot.org/citations/19712110 | http://purl.uniprot.org/core/author | "Muller Y.A."xsd:string |
| http://purl.uniprot.org/citations/19712110 | http://purl.uniprot.org/core/author | "Hillen W."xsd:string |
| http://purl.uniprot.org/citations/19712110 | http://purl.uniprot.org/core/author | "Gmeiner P."xsd:string |
| http://purl.uniprot.org/citations/19712110 | http://purl.uniprot.org/core/author | "Klieber M.A."xsd:string |
| http://purl.uniprot.org/citations/19712110 | http://purl.uniprot.org/core/author | "Lochner S."xsd:string |
| http://purl.uniprot.org/citations/19712110 | http://purl.uniprot.org/core/author | "Scholz O."xsd:string |
| http://purl.uniprot.org/citations/19712110 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19712110 | http://purl.uniprot.org/core/name | "FEBS J"xsd:string |
| http://purl.uniprot.org/citations/19712110 | http://purl.uniprot.org/core/pages | "5610-5621"xsd:string |
| http://purl.uniprot.org/citations/19712110 | http://purl.uniprot.org/core/title | "Structural origins for selectivity and specificity in an engineered bacterial repressor-inducer pair."xsd:string |
| http://purl.uniprot.org/citations/19712110 | http://purl.uniprot.org/core/volume | "276"xsd:string |
| http://purl.uniprot.org/citations/19712110 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19712110 |
| http://purl.uniprot.org/citations/19712110 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19712110 |
| http://purl.uniprot.org/uniprot/#_P04483-mappedCitation-19712110 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19712110 |
| http://purl.uniprot.org/uniprot/#_P0ACT4-mappedCitation-19712110 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19712110 |
| http://purl.uniprot.org/uniprot/P0ACT4 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19712110 |
| http://purl.uniprot.org/uniprot/P04483 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19712110 |