| http://purl.uniprot.org/citations/19720045 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19720045 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19720045 | http://www.w3.org/2000/01/rdf-schema#comment | "The maturation of [NiFe]-hydrogenases is a catalyzed process involving the activities of at least seven proteins. The last step consists of the endoproteolytic cleavage of the precursor of the large subunit, after the [NiFe]-metal center has been assembled. The HycI endopeptidase is involved in the C-terminal processing of HycE, the large subunit of hydrogenase 3 from Escherichia coli. Although HycI has been well characterized biochemically, the crystallization of the protein has been quite challenging. Here, we present the crystal structure of HycI at 1.70 A resolution. The crystal structure resembles the recently reported solution structure (NMR) of the same protein and the holo-HyPD structure of the same family, but a significant conformational change is observed at the L5 loop, as compared with the solution structures of HycI and HyPD. In our crystal structure, three specific metal binding sites (Ca1-3) were identified and these metal ions are possibly involved in the C-terminal cleavage of HycE."xsd:string |
| http://purl.uniprot.org/citations/19720045 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbrc.2009.08.135"xsd:string |
| http://purl.uniprot.org/citations/19720045 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbrc.2009.08.135"xsd:string |
| http://purl.uniprot.org/citations/19720045 | http://purl.uniprot.org/core/author | "Bessho Y."xsd:string |
| http://purl.uniprot.org/citations/19720045 | http://purl.uniprot.org/core/author | "Bessho Y."xsd:string |
| http://purl.uniprot.org/citations/19720045 | http://purl.uniprot.org/core/author | "Kumarevel T."xsd:string |
| http://purl.uniprot.org/citations/19720045 | http://purl.uniprot.org/core/author | "Kumarevel T."xsd:string |
| http://purl.uniprot.org/citations/19720045 | http://purl.uniprot.org/core/author | "Shinkai A."xsd:string |
| http://purl.uniprot.org/citations/19720045 | http://purl.uniprot.org/core/author | "Shinkai A."xsd:string |
| http://purl.uniprot.org/citations/19720045 | http://purl.uniprot.org/core/author | "Tanaka T."xsd:string |
| http://purl.uniprot.org/citations/19720045 | http://purl.uniprot.org/core/author | "Tanaka T."xsd:string |
| http://purl.uniprot.org/citations/19720045 | http://purl.uniprot.org/core/author | "Yokoyama S."xsd:string |
| http://purl.uniprot.org/citations/19720045 | http://purl.uniprot.org/core/author | "Yokoyama S."xsd:string |
| http://purl.uniprot.org/citations/19720045 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19720045 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19720045 | http://purl.uniprot.org/core/name | "Biochem. Biophys. Res. Commun."xsd:string |
| http://purl.uniprot.org/citations/19720045 | http://purl.uniprot.org/core/name | "Biochem. Biophys. Res. Commun."xsd:string |
| http://purl.uniprot.org/citations/19720045 | http://purl.uniprot.org/core/pages | "310-314"xsd:string |
| http://purl.uniprot.org/citations/19720045 | http://purl.uniprot.org/core/pages | "310-314"xsd:string |
| http://purl.uniprot.org/citations/19720045 | http://purl.uniprot.org/core/title | "Crystal structure of hydrogenase maturating endopeptidase HycI from Escherichia coli."xsd:string |
| http://purl.uniprot.org/citations/19720045 | http://purl.uniprot.org/core/title | "Crystal structure of hydrogenase maturating endopeptidase HycI from Escherichia coli."xsd:string |
| http://purl.uniprot.org/citations/19720045 | http://purl.uniprot.org/core/volume | "389"xsd:string |
| http://purl.uniprot.org/citations/19720045 | http://purl.uniprot.org/core/volume | "389"xsd:string |