| http://purl.uniprot.org/citations/19737896 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19737896 | http://www.w3.org/2000/01/rdf-schema#comment | "Cationic amino acids contribute to alpha-defensin bactericidal activity. Curiously, although Arg and Lys have equivalent electropositive charges at neutral pH, alpha-defensins contain an average of nine Arg residues per Lys residue. To investigate the role of high alpha-defensin Arg content, all Arg residues in mouse Paneth cell alpha-defensin cryptdin 4 (Crp4) and rhesus myeloid alpha-defensin 4 (RMAD-4) were replaced with Lys to prepare (R/K)-Crp4 and (R/K)-RMAD-4, respectively. Lys-for-Arg replacements in Crp4 attenuated bactericidal activity and slowed the kinetics of Escherichia coli ML35 cell permeabilization, and (R/K)-Crp4 required longer exposure times to reduce E. coli cell survival. In marked contrast, Lys substitutions in RMAD-4 improved microbicidal activity against certain bacteria and permeabilized E. coli more effectively. Therefore, Arg-->Lys substitutions attenuated activity in Crp4 but not in RMAD-4, and the functional consequences of Arg-->Lys replacements in alpha-defensins are dependent on the peptide primary structure. In addition, the bactericidal effects of (R/K)-Crp4 and (R/K)-RMAD-4 were more sensitive to inhibition by NaCl than those of the native peptides, suggesting that the high Arg content of alpha-defensins may be under selection to confer superior microbicidal function under physiologic conditions."xsd:string |
| http://purl.uniprot.org/citations/19737896 | http://purl.org/dc/terms/identifier | "doi:10.1128/iai.00695-09"xsd:string |
| http://purl.uniprot.org/citations/19737896 | http://purl.uniprot.org/core/author | "Ouellette A.J."xsd:string |
| http://purl.uniprot.org/citations/19737896 | http://purl.uniprot.org/core/author | "Cocco M.J."xsd:string |
| http://purl.uniprot.org/citations/19737896 | http://purl.uniprot.org/core/author | "Weeks C.S."xsd:string |
| http://purl.uniprot.org/citations/19737896 | http://purl.uniprot.org/core/author | "Llenado R.A."xsd:string |
| http://purl.uniprot.org/citations/19737896 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19737896 | http://purl.uniprot.org/core/name | "Infect Immun"xsd:string |
| http://purl.uniprot.org/citations/19737896 | http://purl.uniprot.org/core/pages | "5035-5043"xsd:string |
| http://purl.uniprot.org/citations/19737896 | http://purl.uniprot.org/core/title | "Electropositive charge in alpha-defensin bactericidal activity: functional effects of Lys-for-Arg substitutions vary with the peptide primary structure."xsd:string |
| http://purl.uniprot.org/citations/19737896 | http://purl.uniprot.org/core/volume | "77"xsd:string |
| http://purl.uniprot.org/citations/19737896 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19737896 |
| http://purl.uniprot.org/citations/19737896 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19737896 |
| http://purl.uniprot.org/uniprot/#_P28311-mappedCitation-19737896 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19737896 |
| http://purl.uniprot.org/uniprot/#_Q5I2P9-mappedCitation-19737896 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19737896 |
| http://purl.uniprot.org/uniprot/Q5I2P9 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19737896 |
| http://purl.uniprot.org/uniprot/P28311 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19737896 |