| http://purl.uniprot.org/citations/19737933 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19737933 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19737933 | http://www.w3.org/2000/01/rdf-schema#comment | "Arachidonic acid (AA) is metabolized by endothelial 15-lipoxygenase (15-LO) to several vasodilatory eicosanoids such as 11,12,15-trihydroxyeicosatrienoic acid (11,12,15-THETA) and its proposed unstable precursor 15-hydroxy-11,12-epoxyeicosatrienoic acid (15-H-11,12-EETA). In the present study, the acid-stable 13-hydroxy-trans-14,15-epoxy-eicosatrienoic acid (13-H-14,15-EETA) was identified and its vascular activities characterized. Rabbit aorta, mesenteric arteries, and the combination of 15-LO and cytochrome P450 2J2 converted AA to two distinct HEETA metabolites. The HEETA metabolites were resistant to acidic hydrolysis but were hydrolyzed by recombinant sEH to a more polar metabolite identified by mass spectrometry as 13,14,15-THETA. Mass spectrometric analyses and HPLC comigration identified the HEETAs as threo- and erythro-diastereomers of 13-H-trans-14,15-EETA. Erythro- and threo-diastereomers of 13-H-trans-14,15-EETA relaxed endothelium-denuded rabbit small mesenteric arteries with maximum relaxations of 22.6 +/-6.0% and 8.6 +/-4.3%, respectively. Apamin (10(-7) m) inhibited the relaxations to the erythro-isomer (maximum relaxation = 1.2 +/-5.6%) and increasing [K(+)](o) from 4.6 to 30 mm blocked relaxations to both isomers. In cell-attached patches of mesenteric arterial smooth muscle cells (SMCs), erythro-13-H-trans-14,15-EETA (1-3 x 10(-6) m) increased mean open time of small conductance K(+) channels (13-14 pS) from 0.0007 +/-0.0007 to 0.0053 +/-0.0042. This activation was inhibited by apamin. The erythro, but not the threo, isomer blocked angiotensin II-stimulated aortic SMC migration. These studies demonstrate that 13-H-14,15-EETAs induces vascular relaxation via K(+) channel activation to cause SMC hyperpolarization. Thus, 13-H-14,15-EETA represents a new endothelial factor."xsd:string |
| http://purl.uniprot.org/citations/19737933 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m109.025627"xsd:string |
| http://purl.uniprot.org/citations/19737933 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m109.025627"xsd:string |
| http://purl.uniprot.org/citations/19737933 | http://purl.uniprot.org/core/author | "Falck J.R."xsd:string |
| http://purl.uniprot.org/citations/19737933 | http://purl.uniprot.org/core/author | "Falck J.R."xsd:string |
| http://purl.uniprot.org/citations/19737933 | http://purl.uniprot.org/core/author | "Hammock B.D."xsd:string |
| http://purl.uniprot.org/citations/19737933 | http://purl.uniprot.org/core/author | "Hammock B.D."xsd:string |
| http://purl.uniprot.org/citations/19737933 | http://purl.uniprot.org/core/author | "Campbell W.B."xsd:string |
| http://purl.uniprot.org/citations/19737933 | http://purl.uniprot.org/core/author | "Campbell W.B."xsd:string |
| http://purl.uniprot.org/citations/19737933 | http://purl.uniprot.org/core/author | "Chawengsub Y."xsd:string |
| http://purl.uniprot.org/citations/19737933 | http://purl.uniprot.org/core/author | "Chawengsub Y."xsd:string |
| http://purl.uniprot.org/citations/19737933 | http://purl.uniprot.org/core/author | "Gauthier K.M."xsd:string |
| http://purl.uniprot.org/citations/19737933 | http://purl.uniprot.org/core/author | "Gauthier K.M."xsd:string |
| http://purl.uniprot.org/citations/19737933 | http://purl.uniprot.org/core/author | "Narsimhaswamy D."xsd:string |
| http://purl.uniprot.org/citations/19737933 | http://purl.uniprot.org/core/author | "Narsimhaswamy D."xsd:string |
| http://purl.uniprot.org/citations/19737933 | http://purl.uniprot.org/core/author | "Nithipatikom K."xsd:string |
| http://purl.uniprot.org/citations/19737933 | http://purl.uniprot.org/core/author | "Nithipatikom K."xsd:string |
| http://purl.uniprot.org/citations/19737933 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19737933 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19737933 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/19737933 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/19737933 | http://purl.uniprot.org/core/pages | "31280-31290"xsd:string |
| http://purl.uniprot.org/citations/19737933 | http://purl.uniprot.org/core/pages | "31280-31290"xsd:string |