| http://purl.uniprot.org/citations/19750511 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19750511 | http://www.w3.org/2000/01/rdf-schema#comment | "Tandem affinity purification (TAP) is a method that allows rapid purification of native protein complexes. We developed an improved technique to fuse the fission yeast genes with a TAP tag. Our technique is based on tagging constructs that contain regions homologous to the target gene cloned into vectors carrying a TAP tag. We used this technique to design strategies for TAP-tagging of predicted Schizosaccharomyces pombe genes (http://mendel.imp.ac.at/Pombe_tagging/). To validate the approach, we purified the proteins, which associated with two evolutionarily conserved proteins Swi5 and Sfr1 as well as three protein kinases Ksg1, Orb6 and Sid1."xsd:string |
| http://purl.uniprot.org/citations/19750511 | http://purl.org/dc/terms/identifier | "doi:10.1002/pmic.200800948"xsd:string |
| http://purl.uniprot.org/citations/19750511 | http://purl.uniprot.org/core/author | "Chen Z."xsd:string |
| http://purl.uniprot.org/citations/19750511 | http://purl.uniprot.org/core/author | "Csaszar E."xsd:string |
| http://purl.uniprot.org/citations/19750511 | http://purl.uniprot.org/core/author | "Mechtler K."xsd:string |
| http://purl.uniprot.org/citations/19750511 | http://purl.uniprot.org/core/author | "Ammerer G."xsd:string |
| http://purl.uniprot.org/citations/19750511 | http://purl.uniprot.org/core/author | "Gregan J."xsd:string |
| http://purl.uniprot.org/citations/19750511 | http://purl.uniprot.org/core/author | "Novatchkova M."xsd:string |
| http://purl.uniprot.org/citations/19750511 | http://purl.uniprot.org/core/author | "Cipak L."xsd:string |
| http://purl.uniprot.org/citations/19750511 | http://purl.uniprot.org/core/author | "Rumpf C."xsd:string |
| http://purl.uniprot.org/citations/19750511 | http://purl.uniprot.org/core/author | "Spirek M."xsd:string |
| http://purl.uniprot.org/citations/19750511 | http://purl.uniprot.org/core/author | "Lugmayr W."xsd:string |
| http://purl.uniprot.org/citations/19750511 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19750511 | http://purl.uniprot.org/core/name | "Proteomics"xsd:string |
| http://purl.uniprot.org/citations/19750511 | http://purl.uniprot.org/core/pages | "4825-4828"xsd:string |
| http://purl.uniprot.org/citations/19750511 | http://purl.uniprot.org/core/title | "An improved strategy for tandem affinity purification-tagging of Schizosaccharomyces pombe genes."xsd:string |
| http://purl.uniprot.org/citations/19750511 | http://purl.uniprot.org/core/volume | "9"xsd:string |
| http://purl.uniprot.org/citations/19750511 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19750511 |
| http://purl.uniprot.org/citations/19750511 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19750511 |
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| http://purl.uniprot.org/uniprot/#_P78780-mappedCitation-19750511 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19750511 |