| http://purl.uniprot.org/citations/19751112 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19751112 | http://www.w3.org/2000/01/rdf-schema#comment | "BMP-11/GDF-11 and Myostatin/GDF-8 are both members of the TGF-beta superfamily that can activate SMAD2/3 phosphorylation via the type I receptors ALK4, ALK5, or ALK7. We tested the ability of BMP-11 and Myostatin to promote self-renewal of human embryonic stem cells (hESC) under feeder-free and serum-free culture conditions in short term (1 week) and medium term cultures (10 weeks). We show that hESC cultured in serum-free medium supplemented with either 20 ng/mL Myostatin or 20 ng/mL BMP-11 maintain the colony and cellular morphology of undifferentiated hESC, maintain POU5f1, NANOG, TRA-1-60, and SSEA4 expression, and display increased SMAD2/3 phosphorylation, similar to hESC cultured in mouse embryonic fibroblast feeder-CM or 20 ng/mL Activin-A. The type I TGF-beta receptor inhibitor SB431542 totally inhibited the maintenance activity of both Myostatin or BMP-11 supplemented medium. Our data show that members of the TGF-beta superfamily, other than Activin-A and GDF3, are able to maintain hES cells in an undifferentiated state under feeder free conditions."xsd:string |
| http://purl.uniprot.org/citations/19751112 | http://purl.org/dc/terms/identifier | "doi:10.1089/clo.2009.0024"xsd:string |
| http://purl.uniprot.org/citations/19751112 | http://purl.uniprot.org/core/author | "Pera M.F."xsd:string |
| http://purl.uniprot.org/citations/19751112 | http://purl.uniprot.org/core/author | "Jamshidi P."xsd:string |
| http://purl.uniprot.org/citations/19751112 | http://purl.uniprot.org/core/author | "Wolvetang E.J."xsd:string |
| http://purl.uniprot.org/citations/19751112 | http://purl.uniprot.org/core/author | "Hannan N.R."xsd:string |
| http://purl.uniprot.org/citations/19751112 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19751112 | http://purl.uniprot.org/core/name | "Cloning Stem Cells"xsd:string |
| http://purl.uniprot.org/citations/19751112 | http://purl.uniprot.org/core/pages | "427-435"xsd:string |
| http://purl.uniprot.org/citations/19751112 | http://purl.uniprot.org/core/title | "BMP-11 and myostatin support undifferentiated growth of human embryonic stem cells in feeder-free cultures."xsd:string |
| http://purl.uniprot.org/citations/19751112 | http://purl.uniprot.org/core/volume | "11"xsd:string |
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