| http://purl.uniprot.org/citations/19800350 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19800350 | http://www.w3.org/2000/01/rdf-schema#comment | "The insulin receptor substrate (IRS) family plays important roles in cellular growth, signaling, and survival in the brain. We identified IRS6/Dok-5, a member of the IRS family, also expressed in heart. Dok-5 expression level significantly increased during cardiomyocyte differentiation of P19CL6 cells. To understand the mechanism of Dok-5 gene expression and regulation during cardiomyocyte differentiation, we first mapped the transcription start site of the mouse Dok-5 gene and characterized its promoter regions. Truncation and mutation analysis of the Dok-5 promoter identified the forkhead binding element responsible for the repression of Dok-5 promoter activation. The co-localization of FOXO3a and Dok-5 in the mouse heart allows FOXO3a to be a transcriptional regulator of Dok-5. Electrophoretic mobility shift assay and chromatin immunoprecipitation assay confirmed that FOXO3a could bind to the Dok-5 promoter, accompanied by FOXO3a translocation from the nucleus to cytoplasm. FOXO3a overexpression could inhibit Dok-5 promoter activity. Silencing FOXO3a expression by siRNA upregulated the expression of Dok-5 and enhanced cardiomyocyte differentiation. Moreover, Dok-5 siRNA attenuated cardiomyocyte differentiation. Our results provide the first evidence that FOXO3a, the PI3K/PKB downstream substrate, acts as a transcriptional repressor to inhibit the expression of Dok-5. Dok-5 is involved in cardiomyocyte differentiation by a PI3K/PKB/FOXO3a signaling pathway."xsd:string |
| http://purl.uniprot.org/citations/19800350 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.yjmcc.2009.09.015"xsd:string |
| http://purl.uniprot.org/citations/19800350 | http://purl.uniprot.org/core/author | "Chen Y."xsd:string |
| http://purl.uniprot.org/citations/19800350 | http://purl.uniprot.org/core/author | "Gong Y."xsd:string |
| http://purl.uniprot.org/citations/19800350 | http://purl.uniprot.org/core/author | "Gao J."xsd:string |
| http://purl.uniprot.org/citations/19800350 | http://purl.uniprot.org/core/author | "Liu W."xsd:string |
| http://purl.uniprot.org/citations/19800350 | http://purl.uniprot.org/core/author | "Peng X."xsd:string |
| http://purl.uniprot.org/citations/19800350 | http://purl.uniprot.org/core/author | "Qiang B."xsd:string |
| http://purl.uniprot.org/citations/19800350 | http://purl.uniprot.org/core/author | "Wang C."xsd:string |
| http://purl.uniprot.org/citations/19800350 | http://purl.uniprot.org/core/author | "Yuan J."xsd:string |
| http://purl.uniprot.org/citations/19800350 | http://purl.uniprot.org/core/author | "Xia Q."xsd:string |
| http://purl.uniprot.org/citations/19800350 | http://purl.uniprot.org/core/author | "Wen J."xsd:string |
| http://purl.uniprot.org/citations/19800350 | http://purl.uniprot.org/core/author | "Yin B."xsd:string |
| http://purl.uniprot.org/citations/19800350 | http://purl.uniprot.org/core/author | "Ke Y."xsd:string |
| http://purl.uniprot.org/citations/19800350 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19800350 | http://purl.uniprot.org/core/name | "J Mol Cell Cardiol"xsd:string |
| http://purl.uniprot.org/citations/19800350 | http://purl.uniprot.org/core/pages | "761-769"xsd:string |
| http://purl.uniprot.org/citations/19800350 | http://purl.uniprot.org/core/title | "Dok-5 is involved in cardiomyocyte differentiation through PKB/FOXO3a pathway."xsd:string |
| http://purl.uniprot.org/citations/19800350 | http://purl.uniprot.org/core/volume | "47"xsd:string |
| http://purl.uniprot.org/citations/19800350 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19800350 |
| http://purl.uniprot.org/citations/19800350 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19800350 |
| http://purl.uniprot.org/uniprot/#_D3Z783-mappedCitation-19800350 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19800350 |
| http://purl.uniprot.org/uniprot/#_D3YYP9-mappedCitation-19800350 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19800350 |
| http://purl.uniprot.org/uniprot/#_D3YXX3-mappedCitation-19800350 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19800350 |