| http://purl.uniprot.org/citations/19820702 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19820702 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19820702 | http://www.w3.org/2000/01/rdf-schema#comment | "The anaphase-promoting complex (APC/C), a ubiquitin ligase, is the target of the spindle-assembly checkpoint (SAC), and it ubiquitylates protein substrates whose degradation regulates progress through mitosis. The identity of the ubiquitin-conjugating (E2) enzymes that work with the APC/C is unclear. In an RNA interference (RNAi) screen for factors that modify release from drug-induced SAC activation, we identified the E2 enzyme UBE2S as an APC/C auxiliary factor that promotes mitotic exit. UBE2S is dispensable in a normal mitosis, but its depletion prolongs drug-induced mitotic arrest and suppresses mitotic slippage. In vitro, UBE2S elongates ubiquitin chains initiated by the E2 enzymes UBCH10 and UBCH5, enhancing the degradation of APC/C substrates by the proteasome. Indeed, following release from SAC-induced mitotic arrest, UBE2S-depleted cells neither degrade crucial APC/C substrates, nor silence this checkpoint, whereas bypassing the SAC through BUBR1 depletion or Aurora-B inhibition negates the requirement for UBE2S. Thus, UBE2S functions with the APC/C in a two-step mechanism to control substrate ubiquitylation that is essential for mitotic exit after prolonged SAC activation, providing a new model for APC/C function in human cells."xsd:string |
| http://purl.uniprot.org/citations/19820702 | http://purl.org/dc/terms/identifier | "doi:10.1038/ncb1983"xsd:string |
| http://purl.uniprot.org/citations/19820702 | http://purl.org/dc/terms/identifier | "doi:10.1038/ncb1983"xsd:string |
| http://purl.uniprot.org/citations/19820702 | http://purl.uniprot.org/core/author | "Wu J."xsd:string |
| http://purl.uniprot.org/citations/19820702 | http://purl.uniprot.org/core/author | "Wu J."xsd:string |
| http://purl.uniprot.org/citations/19820702 | http://purl.uniprot.org/core/author | "Russell P."xsd:string |
| http://purl.uniprot.org/citations/19820702 | http://purl.uniprot.org/core/author | "Russell P."xsd:string |
| http://purl.uniprot.org/citations/19820702 | http://purl.uniprot.org/core/author | "Pines J."xsd:string |
| http://purl.uniprot.org/citations/19820702 | http://purl.uniprot.org/core/author | "Pines J."xsd:string |
| http://purl.uniprot.org/citations/19820702 | http://purl.uniprot.org/core/author | "Matsusaka T."xsd:string |
| http://purl.uniprot.org/citations/19820702 | http://purl.uniprot.org/core/author | "Matsusaka T."xsd:string |
| http://purl.uniprot.org/citations/19820702 | http://purl.uniprot.org/core/author | "Mansfeld J."xsd:string |
| http://purl.uniprot.org/citations/19820702 | http://purl.uniprot.org/core/author | "Mansfeld J."xsd:string |
| http://purl.uniprot.org/citations/19820702 | http://purl.uniprot.org/core/author | "Garnett M.J."xsd:string |
| http://purl.uniprot.org/citations/19820702 | http://purl.uniprot.org/core/author | "Garnett M.J."xsd:string |
| http://purl.uniprot.org/citations/19820702 | http://purl.uniprot.org/core/author | "Venkitaraman A.R."xsd:string |
| http://purl.uniprot.org/citations/19820702 | http://purl.uniprot.org/core/author | "Venkitaraman A.R."xsd:string |
| http://purl.uniprot.org/citations/19820702 | http://purl.uniprot.org/core/author | "Godwin C."xsd:string |
| http://purl.uniprot.org/citations/19820702 | http://purl.uniprot.org/core/author | "Godwin C."xsd:string |
| http://purl.uniprot.org/citations/19820702 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19820702 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19820702 | http://purl.uniprot.org/core/name | "Nat. Cell Biol."xsd:string |
| http://purl.uniprot.org/citations/19820702 | http://purl.uniprot.org/core/name | "Nat. Cell Biol."xsd:string |