| http://purl.uniprot.org/citations/19824918 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19824918 | http://www.w3.org/2000/01/rdf-schema#comment | "Trappin-2/Elafin is a serine protease inhibitor that plays a major role as an anti-inflammatory mediator at mucosal surfaces. In addition, Trappin-2/Elafin has antibacterial activity against Gram-positive and Gram-negative bacterial and fungal pathogens. In this study we examined the production of Trappin-2/Elafin by epithelial cells from the human upper and lower female reproductive tract as well as its activity as an anti-human immunodeficiency virus (HIV)-1 molecule. We found that primary uterine, Fallopian tube, cervical and ectocervical epithelial cells produce Trappin-2/Elafin constitutively and that production of Trappin-2/Elafin is enhanced following stimulation with Poly(I:C), especially by the uterine cells. Given the presence of Trappin-2/Elafin in the reproductive tract, we tested the ability of recombinant Trappin-2/Elafin to inhibit HIV-1, an important sexually transmitted pathogen. We found that recombinant Trappin-2/Elafin was able to inhibit both T-cell-tropic X4/IIIB and macrophage-tropic R5/BaL HIV-1 in a dose-dependent manner. The inhibitory activity was observed when virus was incubated with Trappin-2/Elafin but not when Trappin-2/Elafin was added to cells either before infection or after infection. This suggests that the mechanism of inhibition is likely to be a direct interaction between HIV-1 and Trappin-2/Elafin. Additionally, we measured the levels of secreted Trappin-2/Elafin in cervico-vaginal lavages (CVL) from both HIV-positive and HIV-negative women and found that average levels of secreted Trappin-2/Elafin were higher in the CVL from HIV-negative women, although the values did not reach statistical significance. We also found that women at the secretory phase of the menstrual cycle produced more Trappin-2/Elafin in CVL relative to women at the proliferative phase of the menstrual cycle. Our data suggest that Trappin-2/Elafin might be an important endogenous microbicide of the female reproductive tract that is protective against HIV-1."xsd:string |
| http://purl.uniprot.org/citations/19824918 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1365-2567.2009.03165.x"xsd:string |
| http://purl.uniprot.org/citations/19824918 | http://purl.uniprot.org/core/author | "Shen Z."xsd:string |
| http://purl.uniprot.org/citations/19824918 | http://purl.uniprot.org/core/author | "Mayer K."xsd:string |
| http://purl.uniprot.org/citations/19824918 | http://purl.uniprot.org/core/author | "Ghosh M."xsd:string |
| http://purl.uniprot.org/citations/19824918 | http://purl.uniprot.org/core/author | "Cu-Uvin S."xsd:string |
| http://purl.uniprot.org/citations/19824918 | http://purl.uniprot.org/core/author | "Fahey J.V."xsd:string |
| http://purl.uniprot.org/citations/19824918 | http://purl.uniprot.org/core/author | "Wira C.R."xsd:string |
| http://purl.uniprot.org/citations/19824918 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/19824918 | http://purl.uniprot.org/core/name | "Immunology"xsd:string |
| http://purl.uniprot.org/citations/19824918 | http://purl.uniprot.org/core/pages | "207-219"xsd:string |
| http://purl.uniprot.org/citations/19824918 | http://purl.uniprot.org/core/title | "Trappin-2/Elafin: a novel innate anti-human immunodeficiency virus-1 molecule of the human female reproductive tract."xsd:string |
| http://purl.uniprot.org/citations/19824918 | http://purl.uniprot.org/core/volume | "129"xsd:string |
| http://purl.uniprot.org/citations/19824918 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19824918 |
| http://purl.uniprot.org/citations/19824918 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19824918 |
| http://purl.uniprot.org/uniprot/#_P19957-mappedCitation-19824918 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19824918 |
| http://purl.uniprot.org/uniprot/#_Q6LD69-mappedCitation-19824918 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/19824918 |
| http://purl.uniprot.org/uniprot/Q6LD69 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19824918 |
| http://purl.uniprot.org/uniprot/P19957 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/19824918 |