| http://purl.uniprot.org/citations/19827095 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19827095 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19827095 | http://www.w3.org/2000/01/rdf-schema#comment | "True catalases are tyrosine-liganded, usually tetrameric, hemoproteins with subunit sizes of approximately 55-84 kDa. Recently characterized hemoproteins with a catalase-related structure, yet lacking in catalatic activity, include the 40-43 kDa allene oxide synthases of marine invertebrates and cyanobacteria. Herein, we describe the 1.8 A X-ray crystal structure of a 33 kDa subunit hemoprotein from Mycobacterium avium ssp. paratuberculosis (annotated as MAP-2744c), that retains the core elements of the catalase fold and exhibits an organic peroxide-dependent peroxidase activity. MAP-2744c exhibits negligible catalatic activity, weak peroxidatic activity using hydrogen peroxide (20/s) and strong peroxidase activity (approximately 300/s) using organic hydroperoxides as co-substrate. Key amino acid differences significantly impact prosthetic group conformation and placement and confer a distinct activity to this prototypical member of a group of conserved bacterial "minicatalases". Its structural features and the result of the enzyme assays support a role for MAP-2744c and its close homologues in mitigating challenge by a variety of reactive oxygen species."xsd:string |
| http://purl.uniprot.org/citations/19827095 | http://purl.org/dc/terms/identifier | "doi:10.1002/pro.265"xsd:string |
| http://purl.uniprot.org/citations/19827095 | http://purl.org/dc/terms/identifier | "doi:10.1002/pro.265"xsd:string |
| http://purl.uniprot.org/citations/19827095 | http://purl.uniprot.org/core/author | "Gao B."xsd:string |
| http://purl.uniprot.org/citations/19827095 | http://purl.uniprot.org/core/author | "Gao B."xsd:string |
| http://purl.uniprot.org/citations/19827095 | http://purl.uniprot.org/core/author | "Pakhomova S."xsd:string |
| http://purl.uniprot.org/citations/19827095 | http://purl.uniprot.org/core/author | "Pakhomova S."xsd:string |
| http://purl.uniprot.org/citations/19827095 | http://purl.uniprot.org/core/author | "Newcomer M.E."xsd:string |
| http://purl.uniprot.org/citations/19827095 | http://purl.uniprot.org/core/author | "Newcomer M.E."xsd:string |
| http://purl.uniprot.org/citations/19827095 | http://purl.uniprot.org/core/author | "Boeglin W.E."xsd:string |
| http://purl.uniprot.org/citations/19827095 | http://purl.uniprot.org/core/author | "Boeglin W.E."xsd:string |
| http://purl.uniprot.org/citations/19827095 | http://purl.uniprot.org/core/author | "Brash A.R."xsd:string |
| http://purl.uniprot.org/citations/19827095 | http://purl.uniprot.org/core/author | "Brash A.R."xsd:string |
| http://purl.uniprot.org/citations/19827095 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19827095 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19827095 | http://purl.uniprot.org/core/name | "Protein Sci."xsd:string |
| http://purl.uniprot.org/citations/19827095 | http://purl.uniprot.org/core/name | "Protein Sci."xsd:string |
| http://purl.uniprot.org/citations/19827095 | http://purl.uniprot.org/core/pages | "2559-2568"xsd:string |
| http://purl.uniprot.org/citations/19827095 | http://purl.uniprot.org/core/pages | "2559-2568"xsd:string |
| http://purl.uniprot.org/citations/19827095 | http://purl.uniprot.org/core/title | "The structure and peroxidase activity of a 33-kDa catalase-related protein from Mycobacterium avium ssp. paratuberculosis."xsd:string |
| http://purl.uniprot.org/citations/19827095 | http://purl.uniprot.org/core/title | "The structure and peroxidase activity of a 33-kDa catalase-related protein from Mycobacterium avium ssp. paratuberculosis."xsd:string |
| http://purl.uniprot.org/citations/19827095 | http://purl.uniprot.org/core/volume | "18"xsd:string |
| http://purl.uniprot.org/citations/19827095 | http://purl.uniprot.org/core/volume | "18"xsd:string |