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http://purl.uniprot.org/citations/19838300http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/19838300http://www.w3.org/2000/01/rdf-schema#comment"

Background

Ribosomal RNA (rRNA) is a central regulator of cell growth and may control cancer development. A cis noncoding rRNA (nc-rRNA) upstream from the 45S rRNA transcription start site has recently been implicated in control of rRNA transcription in mouse fibroblasts. We investigated whether a similar nc-rRNA might be expressed in human cancer epithelial cells, and related to any genomic characteristics.

Methodology/principal findings

Using quantitative rRNA measurement, we demonstrated that a nc-rRNA is transcribed in human lung epithelial and lung cancer cells, starting from approximately -1000 nucleotides upstream of the rRNA transcription start site (+1) and extending at least to +203. This nc-rRNA was significantly more abundant in the majority of lung cancer cell lines, relative to a nontransformed lung epithelial cell line. Its abundance correlated negatively with total 45S rRNA in 12 of 13 cell lines (P = 0.014). During sequence analysis from -388 to +306, we observed diverse, frequent intercopy single nucleotide polymorphisms (SNPs) in rRNA, with a frequency greater than predicted by chance at 12 sites. A SNP at +139 (U/C) in the 5' leader sequence varied among the cell lines and correlated negatively with level of the nc-rRNA (P = 0.014). Modelling of the secondary structure of the rRNA 5'-leader sequence indicated a small increase in structural stability due to the +139 U/C SNP and a minor shift in local configuration occurrences.

Conclusions/significance

The results demonstrate occurrence of a sense nc-rRNA in human lung epithelial and cancer cells, and imply a role in regulation of the rRNA gene, which may be affected by a +139 SNP in the 5' leader sequence of the primary rRNA transcript."xsd:string
http://purl.uniprot.org/citations/19838300http://purl.org/dc/terms/identifier"doi:10.1371/journal.pone.0007505"xsd:string
http://purl.uniprot.org/citations/19838300http://purl.uniprot.org/core/author"Alvord W.G."xsd:string
http://purl.uniprot.org/citations/19838300http://purl.uniprot.org/core/author"Anderson L.M."xsd:string
http://purl.uniprot.org/citations/19838300http://purl.uniprot.org/core/author"Shiao Y.H."xsd:string
http://purl.uniprot.org/citations/19838300http://purl.uniprot.org/core/author"Fields J.R."xsd:string
http://purl.uniprot.org/citations/19838300http://purl.uniprot.org/core/author"Hwang C.J."xsd:string
http://purl.uniprot.org/citations/19838300http://purl.uniprot.org/core/author"Shapiro B.A."xsd:string
http://purl.uniprot.org/citations/19838300http://purl.uniprot.org/core/author"Gu Y.D."xsd:string
http://purl.uniprot.org/citations/19838300http://purl.uniprot.org/core/author"Kasprzak W."xsd:string
http://purl.uniprot.org/citations/19838300http://purl.uniprot.org/core/author"Leighty R.M."xsd:string
http://purl.uniprot.org/citations/19838300http://purl.uniprot.org/core/author"Lupascu S.T."xsd:string
http://purl.uniprot.org/citations/19838300http://purl.uniprot.org/core/author"Quinones O."xsd:string
http://purl.uniprot.org/citations/19838300http://purl.uniprot.org/core/date"2009"xsd:gYear
http://purl.uniprot.org/citations/19838300http://purl.uniprot.org/core/name"PLoS One"xsd:string
http://purl.uniprot.org/citations/19838300http://purl.uniprot.org/core/pages"e7505"xsd:string
http://purl.uniprot.org/citations/19838300http://purl.uniprot.org/core/title"An intergenic non-coding rRNA correlated with expression of the rRNA and frequency of an rRNA single nucleotide polymorphism in lung cancer cells."xsd:string
http://purl.uniprot.org/citations/19838300http://purl.uniprot.org/core/volume"4"xsd:string
http://purl.uniprot.org/citations/19838300http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/19838300
http://purl.uniprot.org/citations/19838300http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/19838300
http://purl.uniprot.org/uniprot/#_Q15572-mappedCitation-19838300http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19838300
http://purl.uniprot.org/uniprot/#_Q15573-mappedCitation-19838300http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19838300
http://purl.uniprot.org/uniprot/#_O15160-mappedCitation-19838300http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19838300
http://purl.uniprot.org/uniprot/#_P51946-mappedCitation-19838300http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19838300
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