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http://purl.uniprot.org/citations/19844589http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/19844589http://www.w3.org/2000/01/rdf-schema#comment"

Purpose

To investigate if nerve growth factor (NGF) might modulate toll-like receptor (TLR) 4 and 9 expression in primary cultures of vernal keratoconjunctivitis (VKC)-derived conjunctival epithelial cells (VKC-ECs).

Methods

Primary cultures of ECs were established from VKC (n=7) and healthy-control (n=5) conjunctival specimens. Primary untouched and short-term NGF-exposed VKC-ECs were analyzed for molecular (relative real-time PCR) and biochemical (confocal and fluorescence-activated cell sorting analysis of TLR4 and TLR9 expression. Data were compared to their untreated as well as stimulated healthy-control counterparts. Conditioned media were analyzed for interferon (IFN)-gamma, interleukin (IL)-4, IL-10, and IL-12 p40 secretion.

Results

Primary untouched VKC-ECs showed TLR4 increase and TLR9 decrease compared to their healthy-control counterparts. NGF exposure resulted in a strong upregulation of TLR4 and a moderate upregulation of TLR9 in few passages VKC-ECs. Both TLR4 and TLR9 upregulation occurred in a dose-dependent fashion and were supported by biochemical analysis. NGF triggered a dose-response release of IL-10 in VKC-ECs conditioned media, an effect not detected for IL-4, IL-12 p40, and IFN-gamma.

Conclusions

Our data indicate that NGF is able to induce TLR4/TLR9 overexpression in VKC-ECs. These cells exhibited poor IL-4, IL-12 p40, and IFN-gamma responses to NGF, while a significant IL-10 decreased secretion was detected. The different NGF-induced TLR response between VKC and healthy-control conjunctival ECs as well as the different cytokine response might reflect a different pattern of cell activation according to the state of VKC."xsd:string
http://purl.uniprot.org/citations/19844589http://purl.uniprot.org/core/author"Normando E.M."xsd:string
http://purl.uniprot.org/citations/19844589http://purl.uniprot.org/core/author"Micera A."xsd:string
http://purl.uniprot.org/citations/19844589http://purl.uniprot.org/core/author"Bonini S."xsd:string
http://purl.uniprot.org/citations/19844589http://purl.uniprot.org/core/author"Lambiase A."xsd:string
http://purl.uniprot.org/citations/19844589http://purl.uniprot.org/core/author"Stampachiacchiere B."xsd:string
http://purl.uniprot.org/citations/19844589http://purl.uniprot.org/core/author"Bonini S.'"xsd:string
http://purl.uniprot.org/citations/19844589http://purl.uniprot.org/core/date"2009"xsd:gYear
http://purl.uniprot.org/citations/19844589http://purl.uniprot.org/core/name"Mol Vis"xsd:string
http://purl.uniprot.org/citations/19844589http://purl.uniprot.org/core/pages"2037-2044"xsd:string
http://purl.uniprot.org/citations/19844589http://purl.uniprot.org/core/title"Nerve growth factor modulates toll-like receptor (TLR) 4 and 9 expression in cultured primary VKC conjunctival epithelial cells."xsd:string
http://purl.uniprot.org/citations/19844589http://purl.uniprot.org/core/volume"15"xsd:string
http://purl.uniprot.org/citations/19844589http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/19844589
http://purl.uniprot.org/citations/19844589http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/19844589
http://purl.uniprot.org/uniprot/#_P01139-mappedCitation-19844589http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19844589
http://purl.uniprot.org/uniprot/#_Q6LDU8-mappedCitation-19844589http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/19844589
http://purl.uniprot.org/uniprot/P01139http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/19844589
http://purl.uniprot.org/uniprot/Q6LDU8http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/19844589