| http://purl.uniprot.org/citations/19852990 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19852990 | http://www.w3.org/2000/01/rdf-schema#comment | "Liver-expressed antimicrobial peptide 2 (LEAP-2) is a 40-residue cationic peptide originally purified from human blood ultrafiltrate. The native peptide contains two disulfide bonds and is unique regarding its primary structure. Its biological role is not known but a previous study showed that chemically synthesized LEAP-2 exhibited in vitro antimicrobial activities against several Gram-positive bacteria. In order to determine its antimicrobial mode of action, we expressed human recombinant LEAP-2 in Escherichia coli. Circular dichroism spectroscopy and nuclear magnetic resonance analyses showed that the structure of the recombinant peptide was identical to that of the chemically synthesized and oxidized LEAP-2, with two disulfide bonds between Cys residues in relative 1-3 and 2-4 positions. Minimal inhibitory concentration (MIC) of the recombinant human LEAP-2 was determined by a conventional broth dilution assay. It was found to be bactericidal against Bacillus megaterium at a 200microM concentration. Interestingly, the linear LEAP-2 had a greater antimicrobial activity with a MIC value of 12.5microM, which was comparable to that of magainin2. SYTOX Green uptake was used to assess bacterial membrane integrity. Linear LEAP-2 and magainin2 permeabilized B. megaterium membranes with the same efficiency, whereas oxidized LEAP-2 did not induce stain uptake. Binding of the peptides to plasmid DNA was evaluated by gel retardation assays. The DNA-binding efficacy of linear LEAP-2 was three times higher than that of the peptide-containing disulfide bridges. Altogether, these results show that the secondary structure of human LEAP-2 has a profound impact on its antibacterial activity."xsd:string |
| http://purl.uniprot.org/citations/19852990 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.peptides.2009.10.006"xsd:string |
| http://purl.uniprot.org/citations/19852990 | http://purl.uniprot.org/core/author | "Claverol S."xsd:string |
| http://purl.uniprot.org/citations/19852990 | http://purl.uniprot.org/core/author | "Chaignepain S."xsd:string |
| http://purl.uniprot.org/citations/19852990 | http://purl.uniprot.org/core/author | "Garbay B."xsd:string |
| http://purl.uniprot.org/citations/19852990 | http://purl.uniprot.org/core/author | "Schmitter J.M."xsd:string |
| http://purl.uniprot.org/citations/19852990 | http://purl.uniprot.org/core/author | "Odaert B."xsd:string |
| http://purl.uniprot.org/citations/19852990 | http://purl.uniprot.org/core/author | "Bonneu M."xsd:string |
| http://purl.uniprot.org/citations/19852990 | http://purl.uniprot.org/core/author | "Costaglioli P."xsd:string |
| http://purl.uniprot.org/citations/19852990 | http://purl.uniprot.org/core/author | "Dieryck W."xsd:string |
| http://purl.uniprot.org/citations/19852990 | http://purl.uniprot.org/core/author | "Dufourc E.J."xsd:string |
| http://purl.uniprot.org/citations/19852990 | http://purl.uniprot.org/core/author | "Noubhani A."xsd:string |
| http://purl.uniprot.org/citations/19852990 | http://purl.uniprot.org/core/author | "Joucla G."xsd:string |
| http://purl.uniprot.org/citations/19852990 | http://purl.uniprot.org/core/author | "Santarelli X."xsd:string |
| http://purl.uniprot.org/citations/19852990 | http://purl.uniprot.org/core/author | "Hocquellet A."xsd:string |
| http://purl.uniprot.org/citations/19852990 | http://purl.uniprot.org/core/author | "Cabanne C."xsd:string |
| http://purl.uniprot.org/citations/19852990 | http://purl.uniprot.org/core/author | "Le Senechal C."xsd:string |
| http://purl.uniprot.org/citations/19852990 | http://purl.uniprot.org/core/author | "Milenkov M."xsd:string |
| http://purl.uniprot.org/citations/19852990 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/19852990 | http://purl.uniprot.org/core/name | "Peptides"xsd:string |
| http://purl.uniprot.org/citations/19852990 | http://purl.uniprot.org/core/pages | "58-66"xsd:string |
| http://purl.uniprot.org/citations/19852990 | http://purl.uniprot.org/core/title | "Structure-activity relationship of human liver-expressed antimicrobial peptide 2."xsd:string |
| http://purl.uniprot.org/citations/19852990 | http://purl.uniprot.org/core/volume | "31"xsd:string |
| http://purl.uniprot.org/citations/19852990 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19852990 |