| http://purl.uniprot.org/citations/19861239 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19861239 | http://www.w3.org/2000/01/rdf-schema#comment | "The upstream Ggamma-globin gene cAMP response element (G-CRE) was previously shown to play a role in drug-mediated fetal hemoglobin induction. This effect is achieved via p38 mitogen activated protein kinase (MAPK)-dependent CREB1 and ATF-2 phosphorylation and G-CRE transactivation. Since this motif is also a predicted consensus binding site for cJun we extended our analysis to determine the ability of cJun to transactivate gamma-globin through the G-CRE. Using chromatin immunoprecipitation assays we showed comparable in vivo cJun and CREB1 binding to the G-CRE region. Protein-protein interactions were confirmed between cJun/ATF-2 and CREB1/ATF-2 but not between CREB1 and cJun. However, we observed cJun and CREB1 binding to the G-CRE in vitro by electrophoretic mobility shift assay. Promoter pull-down assay followed by sequential western blot analysis confirmed co-localization of cJun, CREB1, and ATF-2 on the G-CRE. To show functional relevance, enforced expression studies with pLen-cJun and a Ggamma-promoter (-1500 to +36) luciferase reporter were completed; we observed a concentration-dependent increase in luciferase activity with pLen-cJun similar to that produced by CREB1 enforced expression. Moreover, the G/A mutation at -1225 in the G-CRE abolished cJun transactivation. Finally, enforced cJun expression in K562 cells and normal primary erythroid progenitors enhanced endogenous gamma-globin gene expression. We conclude that these data indicate that cJun activates the Ggamma-globin promoter via the G-CRE in a manner comparable with CREB1 and propose a model for gamma-globin activation based on DNA-protein interactions in the G-CRE."xsd:string |
| http://purl.uniprot.org/citations/19861239 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bcmd.2009.10.002"xsd:string |
| http://purl.uniprot.org/citations/19861239 | http://purl.uniprot.org/core/author | "Liu L."xsd:string |
| http://purl.uniprot.org/citations/19861239 | http://purl.uniprot.org/core/author | "Balamurugan P."xsd:string |
| http://purl.uniprot.org/citations/19861239 | http://purl.uniprot.org/core/author | "Pace B.S."xsd:string |
| http://purl.uniprot.org/citations/19861239 | http://purl.uniprot.org/core/author | "Kodeboyina S."xsd:string |
| http://purl.uniprot.org/citations/19861239 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/19861239 | http://purl.uniprot.org/core/name | "Blood Cells Mol Dis"xsd:string |
| http://purl.uniprot.org/citations/19861239 | http://purl.uniprot.org/core/pages | "7-15"xsd:string |
| http://purl.uniprot.org/citations/19861239 | http://purl.uniprot.org/core/title | "cJun modulates Ggamma-globin gene expression via an upstream cAMP response element."xsd:string |
| http://purl.uniprot.org/citations/19861239 | http://purl.uniprot.org/core/volume | "44"xsd:string |
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