| http://purl.uniprot.org/citations/19864628 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19864628 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/19864628 | http://www.w3.org/2000/01/rdf-schema#comment | "The ubiquitous cellular influence of nitric oxide (NO) is exerted substantially through protein S-nitrosylation. Whereas NO is highly promiscuous, physiological S-nitrosylation is typically restricted to one or very few Cys residue(s) in target proteins. The molecular basis for this specificity may derive from properties of the target protein, the S-nitrosylating species, or both. Here, we describe a protein microarray-based approach to investigate determinants of S-nitrosylation by biologically relevant low-mass S-nitrosothiols (SNOs). We identify large sets of yeast and human target proteins, among which those with active-site Cys thiols residing at N termini of alpha-helices or within catalytic loops were particularly prominent. However, S-nitrosylation varied substantially even within these families of proteins (e.g., papain-related Cys-dependent hydrolases and rhodanese/Cdc25 phosphatases), suggesting that neither secondary structure nor intrinsic nucleophilicity of Cys thiols was sufficient to explain specificity. Further analyses revealed a substantial influence of NO-donor stereochemistry and structure on efficiency of S-nitrosylation as well as an unanticipated and important role for allosteric effectors. Thus, high-throughput screening and unbiased proteome coverage reveal multifactorial determinants of S-nitrosylation (which may be overlooked in alternative proteomic analyses), and support the idea that target specificity can be achieved through rational design of S-nitrosothiols."xsd:string |
| http://purl.uniprot.org/citations/19864628 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.0900729106"xsd:string |
| http://purl.uniprot.org/citations/19864628 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.0900729106"xsd:string |
| http://purl.uniprot.org/citations/19864628 | http://purl.uniprot.org/core/author | "Forrester M.T."xsd:string |
| http://purl.uniprot.org/citations/19864628 | http://purl.uniprot.org/core/author | "Forrester M.T."xsd:string |
| http://purl.uniprot.org/citations/19864628 | http://purl.uniprot.org/core/author | "Stamler J.S."xsd:string |
| http://purl.uniprot.org/citations/19864628 | http://purl.uniprot.org/core/author | "Stamler J.S."xsd:string |
| http://purl.uniprot.org/citations/19864628 | http://purl.uniprot.org/core/author | "Foster M.W."xsd:string |
| http://purl.uniprot.org/citations/19864628 | http://purl.uniprot.org/core/author | "Foster M.W."xsd:string |
| http://purl.uniprot.org/citations/19864628 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19864628 | http://purl.uniprot.org/core/date | "2009"xsd:gYear |
| http://purl.uniprot.org/citations/19864628 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/19864628 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/19864628 | http://purl.uniprot.org/core/pages | "18948-18953"xsd:string |
| http://purl.uniprot.org/citations/19864628 | http://purl.uniprot.org/core/pages | "18948-18953"xsd:string |
| http://purl.uniprot.org/citations/19864628 | http://purl.uniprot.org/core/title | "A protein microarray-based analysis of S-nitrosylation."xsd:string |
| http://purl.uniprot.org/citations/19864628 | http://purl.uniprot.org/core/title | "A protein microarray-based analysis of S-nitrosylation."xsd:string |
| http://purl.uniprot.org/citations/19864628 | http://purl.uniprot.org/core/volume | "106"xsd:string |
| http://purl.uniprot.org/citations/19864628 | http://purl.uniprot.org/core/volume | "106"xsd:string |
| http://purl.uniprot.org/citations/19864628 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19864628 |
| http://purl.uniprot.org/citations/19864628 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/19864628 |
| http://purl.uniprot.org/citations/19864628 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19864628 |
| http://purl.uniprot.org/citations/19864628 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/19864628 |